An Li-Yun, Li Jun-Jian, Yan Jun-Xia, Li Hong-Jian
Institute of Loess Plateau, Shanxi University, Taiyuan 030006, China.
Huan Jing Ke Xue. 2017 Oct 8;38(10):4420-4426. doi: 10.13227/j.hjkx.201702058.
Soil microbial community plays an important role in ecosystem functions; however, little is known about the importance of microbial diversity to the ecosystems. In this study, serial dilution of soil suspension (10, 10, and 10) was performed and inoculated into the sterilized soils that form the broadleaf mixed forests in Pangquangou. The change in the carbon mineralization rate and the pattern of the carbon source utilization were studied by titration, Biolog Eco, and other experimental methods. The results show that after being incubated for six weeks, carbon mineralization rate, the cumulative amount of carbon mineralization, average well color development (AWCD), and diversity index (Shannon, McIntosh, and richness index) of D1 were significantly higher than those of the D5 treatment. The cumulative amount of carbon mineralization and AWCD was strongly and inversely correlated with richness. Principal component analysis and one-way ANOVA also indicated that the patterns of carbon source utilization of microbially diverse soil were different. Therefore, the loss of microbial diversity affects the carbon mineralization rate and the pattern of carbon source utilization, leading to functional changes in terrestrial ecosystems. In the management of forest soils, the effects of soil microbial diversity on ecosystem functions should be considered.
土壤微生物群落对生态系统功能起着重要作用；然而，人们对微生物多样性对生态系统的重要性知之甚少。在本研究中，对土壤悬浮液进行了系列稀释（10⁻¹、10⁻²和10⁻³），并接种到形成庞泉沟阔叶林的灭菌土壤中。通过滴定法、Biolog Eco等实验方法研究了碳矿化速率的变化和碳源利用模式。结果表明，培养六周后，D1处理的碳矿化速率、碳矿化累积量、平均孔颜色发展（AWCD）和多样性指数（香农指数、麦金托什指数和丰富度指数）均显著高于D5处理。碳矿化累积量和AWCD与丰富度呈显著负相关。主成分分析和单因素方差分析也表明，微生物多样性不同的土壤的碳源利用模式存在差异。因此，微生物多样性的丧失会影响碳矿化速率和碳源利用模式，导致陆地生态系统功能发生变化。在森林土壤管理中，应考虑土壤微生物多样性对生态系统功能的影响。
