Liu Guo-Xin, Wu Hai-Zhen, Sun Sheng-Li, Hu Xiao-Yi, Wu Xiao-Ying, Chen Hua-Yong, Fan Yi-Wen, Hu Cheng-Sheng, Wei Chao-Hai
School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, China.
Guangzhou Municipal Engineering Maintenance Department, Guangzhou 510000, China.
Huan Jing Ke Xue. 2017 Sep 8;38(9):3807-3815. doi: 10.13227/j.hjkx.201702146.
Coking wastewater is a typical industrial wastewater with high toxicity. Its treatment with biological processes is often challenging because it contains constituents inhibiting microbial activity. To study the inhibitory effect and possible acclimation of microbes in coking wastewater treatment, municipal sludge was inoculated into coking wastewater. Time-dependent concentrations of COD, phenol, ammonia nitrogen, and thiocyanide in coking wastewater were analyzed. The microbial community structure was investigated by the Illumina high-throughput sequencing technology during inoculation. The results showed that COD began to decrease after 16 h and 97.1% of phenol disappeared after 40 h. Thiocyanide began to degrade at 72 h and was undetectable after 96 h. Accordingly, the concentration of ammonia increased as the thiocyanide concentrations decreased. High-throughput pyrosequencing analysis showed that the microbial community structure and species richness varied at different culture stages. In the stage of phenol degradation, the abundance of and increased rapidly; the species richness was 13.04% of the community at 48 h. In the stage of thiocyanate degradation, , and were the dominant bacteria and were 16.13% of the community at 96 h. At 144 h, and became the dominant species and were 22.45% of the community abundance. The results showed that municipal sludge can rapidly overcome the toxicity of coking wastewater because the pollutants are degraded rapidly. The microbial community structure changed as wastewater components were degraded. Environmental factors and the competition among bacteria played a key role in microbial community succession.
焦化废水是一种典型的高毒性工业废水。采用生物法处理该废水往往具有挑战性，因为其所含成分会抑制微生物活性。为研究焦化废水处理中微生物的抑制作用及可能的驯化情况，将城市污泥接种到焦化废水中。分析了焦化废水中化学需氧量（COD）、苯酚、氨氮和硫氰化物随时间变化的浓度。在接种过程中，采用Illumina高通量测序技术研究微生物群落结构。结果表明，16小时后COD开始下降，40小时后97.1%的苯酚消失。硫氰化物在72小时开始降解，96小时后检测不到。相应地，随着硫氰化物浓度降低，氨的浓度增加。高通量焦磷酸测序分析表明，在不同培养阶段微生物群落结构和物种丰富度有所不同。在苯酚降解阶段， 和 的丰度迅速增加；48小时时物种丰富度占群落的13.04%。在硫氰酸盐降解阶段， 、 和 是优势细菌，96小时时占群落的16.13%。144小时时， 和 成为优势物种，占群落丰度的22.45%。结果表明，城市污泥能够迅速克服焦化废水的毒性，因为污染物降解迅速。随着废水成分的降解，微生物群落结构发生变化。环境因素和细菌间的竞争在微生物群落演替中起关键作用。
