[Rapid Culture, Microbial Community Structure, and Diversity of High-Efficiency Denitrifying Bacteria].

High-efficiency denitrifying bacteria can improve wastewater treatment efficiency. In order to rapidly culture high-efficiency denitrifying bacteria, batch trials were conducted at different carbon sources (No. 1 and No. 2 fermented liquid) and NO-N concentration gradients (30 mg·L-150 mg·L-300 mg·L). The results showed that No. 2 fermented liquid could finish high-efficiency denitrifying bacteria[300 mg·(L·h)] cultivation in 11 d, which is 17 d before No.1 fermented liquid could. At the same time, high-efficiency denitrifying bacteria could effectively remove ammonia-nitrogen and total phosphorus, and maximum removal rates were 34.43 mg·(L·h) and 2.98 mg·(L·h), respectively. Furthermore, high-throughput sequencing was employed to characterize and compare the community structure and diversity. The species abundance and diversity were reduced but the category and proportion of denitrifying bacteria were increased. In addition, bacteria composition and quantity changed after cultivation and and were the dominant contributors to the effective removal of nitrate in the SBR(sequencing batch reactor). Denitrifying phosphorus accumulating bacteria (Rhodocyclaceae and Pseudomonadaceae) and heterotrophic nitrifying bacteria ( and ) proved the capacity of the system to remove ammonia-nitrogen and total phosphorus.