[Isolation, Identification and Characterization of the Filamentous Microorganisms from Bulking Sludge].

To illustrate the species and characteristics of filamentous microorganisms in bulking sludge, culture method, microscopic examination and molecular biological analysis were conducted to isolate and identify the filamentous microorganisms existing in bulking sludge collected from municipal wastewater treatment plant. Filamentous microorganisms isolated by Gause's synthetic agar medium and starch agar fell into 18 genera, among which and belong to bacterial phylum Actinobacteria, all other isolates were classified into fungi. and were the fungi with high occurrence frequency in culture medium. and could grow well at neutral pH or meta-acid condition. High concentration of NaCl could inhibit the growth of and , but had no obvious inhibitory effect on and . Except of and all could utilize saccharose, starch and cellulose. The increase of carbon source concentration could promote their growth. The results of high throughput sequencing of rDNA-ITS showed that a large number of unidentified fungi existed in bulking sludge.