Li Huan, Wang Lei, Wang Ya-Nan
State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China.
Huan Jing Ke Xue. 2017 Jun 8;38(6):2496-2501. doi: 10.13227/j.hjkx.201610043.
The effect of NaSO·5HO on the carbon fixation capability of (DSM 505) was determined by measuring the total organic carbon (TOC) concentrations under different concentrations of NaSO·5HO. In addition, the mechanism was clarified by analyzing the transcription characteristics of RubisCO-encoding genes ( genes) and the concentrations of extracellular free organic carbon (EFOC) under different concentrations of NaSO·5HO. The result showed that by increasing the concentrations of NaSO·5HO appropriately, the carbon fixation capability of could be promoted and the ratio of extracellular free organic carbon to total organic carbon fell significantly. Moreover, the analysis from the transcription characteristics of genes revealed that NaSO·5HO had no significant effect on the transcription efficiency and transcription pattern of genes. So NaSO·5HO might improve the carbon fixation capability by promoting the cytoskeleton synthesis rate as electron donors to eliminate the inhibition effect of extracellular free organic carbon on the carbon fixation of .
通过测量不同浓度的Na₂SO₄·5H₂O下的总有机碳（TOC）浓度，确定了Na₂SO₄·5H₂O对（DSM 505）碳固定能力的影响。此外，通过分析不同浓度的Na₂SO₄·5H₂O下核酮糖-1,5-二磷酸羧化酶/加氧酶编码基因（rbcL基因）的转录特征和细胞外游离有机碳（EFOC）的浓度，阐明了其作用机制。结果表明，适当提高Na₂SO₄·5H₂O的浓度，可以促进（DSM 505）的碳固定能力，细胞外游离有机碳与总有机碳的比例显著下降。此外，对rbcL基因转录特征的分析表明，Na₂SO₄·5H₂O对rbcL基因的转录效率和转录模式没有显著影响。因此，Na₂SO₄·5H₂O可能通过促进细胞骨架合成速率作为电子供体来消除细胞外游离有机碳对（DSM 505）碳固定的抑制作用，从而提高碳固定能力。
