Qiao Sha-Sha, Zhou Yong-Na, Chai Bao-Feng, Jia Tong, Li Cui
Institute of Loess Plateau, Shanxi University, Taiyuan 030006, China.
Department of Environment and Economics, Shanxi University of Finance and Economics, Taiyuan 030006, China.
Huan Jing Ke Xue. 2017 Jun 8;38(6):2502-2512. doi: 10.13227/j.hjkx.201611101.
Soil microorganisms drive the biogeochemical process of carbon, nitrogen, phosphorus and sulfur, and play a key role in maintaining soil carbon sink and ecosystem function. The study on effects of environmental and spatial factors on the structure of microbial community in boreal coniferous forest soil will provide theoretical basis for making management measures in local forest ecosystem. Our research analyzed five soil fungi communities (LpMC1, LpMC2, PwMC, PtMC, and BMC) in four forest types, including forest, forest, forest and spp. forest, respectively, in Pangquangou Nature Reserve in Guandi Mountains with Illumina high-throughput sequencing technology. Meanwhile, soil environmental factors and diversity of undergrowth plants were determined to analyze the relationship between fungi community structure and vegetation as well as soil environmental factors. The results showed that:①There were seven eumycota and thirty-three advantageous fungal genera in the five sample sites; ②Redundancy analysis results showed that soil pH, temperature, moisture, total nitrogen, the content of NH, total carbon, invertase activity, urease activity, undergrowth dominance and evenness were significantly associated with soil fungi community structure; ③Cluster analysis and principal component analysis showed that forest vegetation type, soil environmental factors and undergrowth had significant effects on soil fungi community structure; ④The results of PCNM analysis showed that at a local scale, dispersal limitation had no significant influence on fungi community structure in the study area. The forest soil fungi community structure in the study area was significantly affected by environmental selection (soil pH, temperature, moisture, total nitrogen, the content of NH, total carbon, invertase activity, urease activity, undergrowth dominance and evenness, forest type).
土壤微生物驱动着碳、氮、磷和硫的生物地球化学过程，在维持土壤碳汇和生态系统功能方面发挥着关键作用。研究环境和空间因素对北方针叶林土壤微生物群落结构的影响，将为制定当地森林生态系统的管理措施提供理论依据。我们的研究利用Illumina高通量测序技术，分析了关帝山庞泉沟自然保护区四种森林类型（分别为 森林、 森林、 森林和 spp.森林）中的五个土壤真菌群落（LpMC1、LpMC2、PwMC、PtMC和BMC）。同时，测定了土壤环境因素和林下植物多样性，以分析真菌群落结构与植被以及土壤环境因素之间的关系。结果表明：①五个采样点共有七个真真菌门和三十三个优势真菌属；②冗余分析结果表明，土壤pH值、温度、湿度、总氮、NH含量、总碳、转化酶活性、脲酶活性、林下优势度和均匀度与土壤真菌群落结构显著相关；③聚类分析和主成分分析表明，森林植被类型、土壤环境因素和林下植被对土壤真菌群落结构有显著影响；④PCNM分析结果表明，在局部尺度上，扩散限制对研究区域内的真菌群落结构没有显著影响。研究区域内的森林土壤真菌群落结构受环境选择（土壤pH值、温度、湿度、总氮、NH含量、总碳、转化酶活性、脲酶活性、林下优势度和均匀度、森林类型）的显著影响。
