Veldhuis J D, Hewlett E L
Biochem Biophys Res Commun. 1985 Sep 30;131(3):1168-74. doi: 10.1016/0006-291x(85)90213-x.
Incubation of particulate fractions of swine granulosa cells or luteal minces with purified pertussis toxin (islet-activating protein) and [32P]-NAD catalyzed the (32P)-ADP ribosylation of a 41,000 dalton membrane protein. ADP-ribosylation was markedly reduced by prior incubation of intact cells with toxin. The functional relevance of this presumptive inhibitory guanine nucleotide-binding protein in pig granulosa cells was indicated by the ability of prior treatment with pertussis toxin to increase cyclic AMP generation and progesterone production significantly in response to follicle stimulating hormone. Prior cellular intoxication also enhanced cyclic AMP production stimulated by luteinizing hormone and choleratoxin, but not basally or after forskolin. These results demonstrate the presence of an inhibitory guanine nucleotide-binding protein in both the follicular (granulosa cell) and luteal compartments of the mammalian ovary, and indicate its functional relevance in cyclic AMP generation and progesterone secretion.
用纯化的百日咳毒素（胰岛激活蛋白）和[32P]-NAD孵育猪颗粒细胞或黄体切碎物的微粒部分，可催化一种41,000道尔顿膜蛋白的（32P）-ADP核糖基化。完整细胞先用毒素孵育会使ADP-核糖基化显著降低。猪颗粒细胞中这种假定的抑制性鸟嘌呤核苷酸结合蛋白的功能相关性，通过预先用百日咳毒素处理能显著增加对促卵泡激素反应的环磷酸腺苷生成和孕酮分泌得以体现。预先的细胞中毒还增强了促黄体生成素和霍乱毒素刺激的环磷酸腺苷生成，但对基础水平或福斯高林刺激后的环磷酸腺苷生成没有影响。这些结果证明哺乳动物卵巢的卵泡（颗粒细胞）和黄体部分均存在抑制性鸟嘌呤核苷酸结合蛋白，并表明其在环磷酸腺苷生成和孕酮分泌中的功能相关性。
