Gao Jing-Feng, Wang Shi-Jie, Fan Xiao-Yan, Pan Kai-Ling, Zhang Li-Fang, Zhang Shu-Jun, Gao Yong-Qing, Zhang Shuai
National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, College of Environmental and Energy Engineering, Beijing University of Technology, Beijing 100124, China.
Research and Development Center of Beijing Drainage Group Co., Ltd., Beijing 100124, China.
Huan Jing Ke Xue. 2017 Nov 8;38(11):4696-4705. doi: 10.13227/j.hjkx.201703275.
In this study, domestic sewage was utilized to cultivate aerobic granular sludge (AGS) in a simultaneous nitrogen and phosphorus removal (SNPR) system. The bacterial population dynamics during the aerobic sludge granulation were investigated to reveal the granulation mechanisms using Illumina MiSeq PE300 high-throughput sequencing. Quantitative real time polymerase chain reactions (PCR) were used to investigate shifts in the abundance of ammonia-oxidizing bacteria (AOB), ammonia-oxidizing archaea (AOA), nitrite-oxidizing bacteria (NOB) and polyphosphate accumulating organisms (PAOs). After cultivation for 100 d, the AGS was compact and demonstrated good SNPR performance. During the AGS formation process, extracellular polysaccharides obviously increased, while extracellular proteins kept relatively stable. The abundance of AOA significantly decreased during the formation of AGS process, while the abundance of PAOs increased. The bacterial diversity increased at first and then decreased during the formation of AGS. The bacterial community changed dramatically during aerobic sludge granulation. Persistent operational taxonomic units (OTUs) accounted for 92.70% of the total sequences. Proteobacteria (31.07%-53.67%), Bacteroidetes (6.70%-16.50%) and Chloroflexi (7.84%-13.36%) were the dominant phyla. was obviously enriched in the AGS formation process (increased from 0.11% in the seed sludge to 35.33% in the AGS) and may play an important role in the formation of AGS.
在本研究中，利用生活污水在同步脱氮除磷（SNPR）系统中培养好氧颗粒污泥（AGS）。采用Illumina MiSeq PE300高通量测序技术研究好氧污泥颗粒化过程中的细菌种群动态，以揭示颗粒化机制。运用定量实时聚合酶链反应（PCR）研究氨氧化细菌（AOB）、氨氧化古菌（AOA）、亚硝酸盐氧化细菌（NOB）和聚磷菌（PAO）丰度的变化。培养100 d后，AGS结构紧密，展现出良好的同步脱氮除磷性能。在AGS形成过程中，胞外多糖明显增加，而胞外蛋白保持相对稳定。在AGS形成过程中，AOA的丰度显著下降，而PAO的丰度增加。在AGS形成过程中，细菌多样性先增加后减少。好氧污泥颗粒化过程中细菌群落发生了显著变化。持续存在的操作分类单元（OTU）占总序列的92.70%。变形菌门（31.07%-53.67%）、拟杆菌门（6.70%-16.50%）和绿弯菌门（7.84%-13.36%）是优势菌门。 在AGS形成过程中明显富集（从种子污泥中的0.11%增加到AGS中的35.33%），可能在AGS形成中起重要作用。
