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太湖竺山湾春季浮游细菌的群落结构及影响因素

[Community Structure and Influencing Factors of Bacterioplankton in Spring in Zhushan Bay, Lake Taihu].

作者信息

Xue Yin-Gang, Liu Fei, Sun Meng, Jiang Xiao-Dong, Geng Jin-Ju, Teng Jia-Quan, Xie Wen-Li, Zhang Hao, Chen Xin-Yi

机构信息

School of Environmental and Safety Engineering, Changzhou University, Changzhou 213164, China.

Key Laboratory of Environmental Protection of Water Environment Biological Monitoring of Jiangsu Province, Changzhou Environmental Monitoring Center, Changzhou 213001, China.

出版信息

Huan Jing Ke Xue. 2018 Mar 8;39(3):1151-1158. doi: 10.13227/j.hjkx.201707164.

Abstract

In order to investigate the characteristics of bacterioplankton in the spring in Zhushan Bay, Lake Taihu, the 16S rRNA gene of the bacterioplankton at four sampling sites in Zhushan Bay was sequenced by high-throughput sequencing using water samples collected from Yapugang, Shatanggang, Zhushanhunan, and Jiaoshan. The results showed that the coverage of the sequencing library was very high and could accurately represent the bacterioplankton community in the samples. The species richness of Jiaoshan was the highest, but the species evenness was lower. Cyanobacteria, Actinobacteria, Proteobacteria, and Bacteroidetes were the dominant phylum in Zhushan Bay. The average abundance of Cyanobacteria was as high as 64.73%, which indicated an outbreak of cyanobacteria bloom in the water. At the genus level, , and were predominant. The results of redundancy analysis (RDA) for the relationship between bacterioplankton and environmental factors showed that water temperature, chlorophyll a (Chl-a), ammonia nitrogen(NH-N), dissolved oxygen (DO), and phosphate (PO-P)were the main environmental factors affecting the bacterioplankton community. Dissolved oxygen could significantly affect ; nutrient and water temperature also had an effect.

摘要

为了研究太湖竺山湾春季浮游细菌的特征,利用从雅浦港、沙塘港、竺山湖南和焦山采集的水样,通过高通量测序对竺山湾4个采样点的浮游细菌16S rRNA基因进行了测序。结果表明,测序文库的覆盖率很高,能够准确代表样本中的浮游细菌群落。焦山的物种丰富度最高,但物种均匀度较低。蓝细菌、放线菌、变形菌和拟杆菌是竺山湾的优势门类。蓝细菌的平均丰度高达64.73%,表明水体中蓝藻爆发。在属水平上, 、 和 占主导地位。浮游细菌与环境因子关系的冗余分析(RDA)结果表明,水温、叶绿素a(Chl-a)、氨氮(NH-N)、溶解氧(DO)和磷酸盐(PO-P)是影响浮游细菌群落的主要环境因子。溶解氧能显著影响 ;营养物质和水温也有影响。

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