Zhu Jin-Shan, Zhang Hui, Ma Lian-Jie, Liao Dun-Xiu, Yang Xing-Yong, Wang Long-Chang, Wang Ding-Yong
Research Center for Environmental Monitoring, Hazard Prevention of Three Gorges Reservoir, Yangtze Normal University, Chongqing 408100, China.
Collaborative Innovation Center for Green Development in Wuling Mountain Areas, Yangtze Normal University, Chongqing 408100, China.
Huan Jing Ke Xue. 2018 May 8;39(5):2400-2411. doi: 10.13227/j.hjkx.201708085.
In order to explore the variation in soil microbial community diversity in paddy fields with different irrigation periods, we collected in situ rice field soils during different biogas irrigation periods and analyzed the microbial community structures of these soils by high-throughput sequencing. The results showed that as the biogas irrigation period increased, the soil pH decreased gradually, while organic matter, nitrate nitrogen, phosphate, and other nutrients were accumulated. Years of continued biogas irrigation was not conducive to improving rice yields. The results showed that as the biogas irrigation period increased, the richness in microbial species in paddy soils decreased gradually, and the diversity in the microbial communities was also reduced. Proteobacteria accounts for the largest proportion in rice paddy soil with biogas slurry irrigation. With the increase of biogas irrigation years, the proportion of -Proteobacteria, Bacteroidia, Bacteroidales, Burkholderiales, , and increased, while the proportion of Gemmatimonadetes and -Proteobacteria decreased gradually. Dissolved organic carbon (=2.67, =0.09) had the greatest effect on microbial community structures in the studied paddy soils.
为了探究不同灌溉时期稻田土壤微生物群落多样性的变化，我们在不同沼液灌溉时期采集了稻田原位土壤，并通过高通量测序分析了这些土壤的微生物群落结构。结果表明，随着沼液灌溉时期的增加，土壤pH值逐渐降低，而有机质、硝态氮、磷酸盐等养分则有所积累。多年持续沼液灌溉不利于提高水稻产量。结果表明，随着沼液灌溉时期的增加，稻田土壤中微生物物种的丰富度逐渐降低，微生物群落的多样性也随之减少。在有沼液灌溉的稻田土壤中，变形菌门占比最大。随着沼液灌溉年限的增加，δ-变形菌纲、拟杆菌纲、拟杆菌目、伯克氏菌目等的占比增加，而芽单胞菌门和α-变形菌纲的占比则逐渐降低。溶解性有机碳（r = 2.67，P = 0.09）对所研究稻田土壤的微生物群落结构影响最大。
