Yi Jun, Cheng Jin-Ping
State Key Laboratory of Estuarine and Coastal Research, East China Normal University, Shanghai 200062, China.
Shenzhen Key Laboratory for the Sustainable Use of Marine Biodiversity, Research Centre for the Oceans and Human Health, City University of Hong Kong Shenzhen Research Institute, Shenzhen 518057, China.
Huan Jing Ke Xue. 2017 Mar 8;38(3):1173-1181. doi: 10.13227/j.hjkx.201608182.
This study aimed to understand how the physicochemical properties of silver nanoparticles (AgNPs) affected its toxicity in the aquatic environment. The morphologies of oleylamine-coated AgNPs (4 nm) under environmental and laboratory water conditions were studied under transmission electron microscopy, and the results showed oleylamine-coated AgNPs formed big aggregates in the environmental water samples after 12 h equilibration. Dynamic light scattering results also showed that oleylamine-coated AgNPs had larger hydrodynamic size in all water samples as compared to its primary size (4 nm). Exposure to oleylamine-coated AgNPs inhibited the growth of () and reduced its cell viability in Luria-Bertani (LB) broth and 0.85% NaCl, respectively. A dose-dependent toxicity of AgNPs in was observed in laboratorial medium conditions but not in environmental water condition. Low concentration of AgNPs exhibited lower toxicity in environmental water samples as compared to that in laboratorial medium conditions. Separating AgNPs from bacterial suspension through a dialysis membrane enhanced the cell viability of significantly. In the environmental water samples, the AgNPs-bacteria interaction would be reduced with the formation of big aggregates by AgNPs, which consequently decreased its toxicity in aquatic environment.
本研究旨在了解银纳米颗粒（AgNPs）的物理化学性质如何影响其在水生环境中的毒性。在透射电子显微镜下研究了油胺包覆的AgNPs（4纳米）在环境水和实验室用水条件下的形态，结果表明，油胺包覆的AgNPs在环境水样中平衡12小时后形成了大的聚集体。动态光散射结果还表明，与原始尺寸（4纳米）相比，油胺包覆的AgNPs在所有水样中的流体动力学尺寸更大。暴露于油胺包覆的AgNPs分别抑制了（）的生长并降低了其在Luria-Bertani（LB）肉汤和0.85%NaCl中的细胞活力。在实验室培养基条件下观察到AgNPs对（）具有剂量依赖性毒性，但在环境水条件下未观察到。与实验室培养基条件相比，低浓度的AgNPs在环境水样中表现出较低的毒性。通过透析膜将AgNPs与细菌悬浮液分离可显著提高（）的细胞活力。在环境水样中，AgNPs形成大聚集体会减少AgNPs与细菌的相互作用，从而降低其在水生环境中的毒性。
