State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, PR China; University of the Chinese Academy of Sciences, Beijing 100049, PR China.
Talanta. 2013 Dec 15;117:449-55. doi: 10.1016/j.talanta.2013.09.017. Epub 2013 Oct 3.
A simple method based on electrostatic interactions was utilized to assemble silver nanoparticles (AgNPs) to graphene oxide (GO) sheets. This method allows conjugation of AgNPs with desired morphologies (densities, sizes and shapes) onto GO. In this process, poly(diallyldimethylammonium chloride) (PDDA) was introduced as an adhesive agent. The as-prepared graphene oxide-AgNPs composites (GO-AgNPs) have enhanced colloid stability and photo-stability than that of AgNPs. After conjugating to GO sheets, the antibacterial activities of AgNPs against Gram negative (G-) bacterial strain (Escherichia coli, E. coli) and Gram positive (G+) bacterial strain (Bacillus subtilis, B. subtilis) have been improved significantly. The antibacterial activity of GO-AgNPs is dependent on the size of AgNPs, i.e. the small AgNPs modified GO sheets show more effective antibacterial capability than that of large AgNPs modified GO sheets. Compared with AgNPs, the enhanced antibacterial activity of GO-AgNPs might not only be due to high stability of AgNPs anchored on GO sheets, but also the positive charged surface of hybrids which increases the electrostatic interaction of bacterial cell membrane with nanohybrids.
一种基于静电相互作用的简单方法被用于将银纳米粒子 (AgNPs) 组装到氧化石墨烯 (GO) 片上。这种方法允许具有所需形态（密度、大小和形状）的 AgNPs 与 GO 进行共轭。在这个过程中，聚二烯丙基二甲基氯化铵 (PDDA) 被引入作为一种粘合剂。与 GO 片共轭后，与 AgNPs 相比，氧化石墨烯-银纳米粒子复合材料 (GO-AgNPs) 具有增强的胶体稳定性和光稳定性。GO-AgNPs 的抗菌活性对革兰氏阴性 (G-) 细菌菌株（大肠杆菌，E. coli）和革兰氏阳性 (G+) 细菌菌株（枯草芽孢杆菌，B. subtilis）有显著提高。GO-AgNPs 的抗菌活性取决于 AgNPs 的尺寸，即经过修饰的小 AgNPs 的 GO 片显示出比大 AgNPs 修饰的 GO 片更有效的抗菌能力。与 AgNPs 相比，GO-AgNPs 的增强抗菌活性可能不仅归因于 AgNPs 在 GO 片上的高稳定性，还归因于杂交物的带正电荷的表面增加了纳米杂化物与细胞膜之间的静电相互作用。
