USDA-ARS, U.S. Salinity Laboratory, 450 W. Big Springs Rd, Riverside, CA 92507, USA.
Department of Animal and Veterinary Sciences, California State Polytechnic University, Pomona, CA 91768, USA.
Int J Environ Res Public Health. 2018 Jun 29;15(7):1369. doi: 10.3390/ijerph15071369.
The microbiological quality of treated waste water is always a concern when waste water is disposed to the environment. However, when treated appropriately, such water can serve many purposes to the general population. Therefore, the treatment and removal of contaminants from swine waste water by continuous flow-constructed wetlands involves complex biological, physical, and chemical processes that may produce better quality water with reduced levels of contaminants. Swine waste contains populations and other bacterial contaminants originating from swine houses through constructed wetlands, but little is known about population in swine waste water. To assess the impacts of seasonal variations and the effect of the wetland layout/operations on water quality, isolates were compared for genetic diversity using repetitive extragenic palindromic polymerase chain reaction (REP-PCR). None of the isolates was confirmed as Shiga toxin producing O157:H7 (STEC); however, other pathotypes, such as enterotoxigenic (ETEC) were identified. Using a 90% similarity index from REP-PCR, 69 genotypes out of 421 isolates were found. Our data showed that the population was significantly ( = 0.036) higher in November than in March and August in most of the wetland cells. Furthermore, there was a significant ( = 0.001) reduction in populations from wetland influent to the final effluent. Therefore, the use of continuous flow-constructed wetlands may be a good treatment approach for reducing contaminants from different waste water sources.
处理后的废水的微生物质量一直是人们关注的焦点，特别是当废水被排放到环境中时。然而，当废水得到适当处理时，它可以为大众提供多种用途。因此，通过连续流人工湿地处理和去除猪废水中的污染物涉及复杂的生物、物理和化学过程，可能会产生更好质量且污染物含量更低的水。猪废水中含有大量的种群和其他细菌污染物，这些污染物来源于猪舍，但对于猪废水中的种群知之甚少。为了评估季节性变化和湿地布局/操作对水质的影响，使用重复基因外回文序列聚合酶链反应（REP-PCR）比较了遗传多样性的分离株。虽然没有分离株被确认为产志贺毒素的 O157：H7（STEC），但鉴定出了其他的病原体，如肠毒性（ETEC）。使用 REP-PCR 的 90%相似性指数，从 421 个分离株中发现了 69 种基因型。我们的数据表明，在大多数湿地单元中，11 月的种群数量明显（=0.036）高于 3 月和 8 月。此外，从湿地进水到最终出水，种群数量显著减少（=0.001）。因此，连续流人工湿地的使用可能是减少不同废水来源中污染物的一种很好的处理方法。
