从携带重组质粒的菌株中过量生产和纯化起始因子IF2和pNUSA蛋白。
Overproduction and purification of initiation factor IF2 and pNUSA proteins from a recombinant plasmid bearing strain.
作者信息
Dondon J, Plumbridge J A, Hershey J W, Grunberg-Manago M
出版信息
Biochimie. 1985 Jun;67(6):643-9. doi: 10.1016/s0300-9084(85)80206-6.
PMID:2996631
Abstract
The genes for translational initiation factor, IF2 and pNusA have been cloned into a plasmid vector where they are placed under the control of the inducible lambdapL promoter and the c1857 thermosensitive repressor. When a strain carrying this plasmid is heat induced, IF2 alpha, IF2 beta and pNusA are overproduced 15 to 20 fold. This has allowed us to purify the IF2 and NusA proteins in large amounts.
摘要
翻译起始因子IF2和pNusA的基因已被克隆到一个质粒载体中，它们在可诱导的λpL启动子和c1857温度敏感阻遏物的控制下。当携带该质粒的菌株受热诱导时，IF2α、IF2β和pNusA的产量会增加15至20倍。这使我们能够大量纯化IF2和NusA蛋白。
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