调查假单胞菌属细菌菌株的 O-多糖作为噬菌体 BIM BV-45 的潜在受体。

Investigation of O-polysaccharides from bacterial strains of Pseudomonas genus as potential receptors of bacteriophage BIM BV-45.

机构信息

N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation.

Institute of Microbiology, National Academy of Sciences of Belarus, 220141 Minsk, Belarus.

出版信息

Int J Biol Macromol. 2018 Oct 15;118(Pt A):1065-1072. doi: 10.1016/j.ijbiomac.2018.06.165. Epub 2018 Jun 30.

DOI:10.1016/j.ijbiomac.2018.06.165

PMID:29966669

Abstract

The structure of potential bacteriophage receptors located on cell walls of Gram-negative bacteria deposited at Belarusian collection of microorganisms was investigated. Studies by 1D and 2D H and C NMR spectroscopy enabled to elucidate the structure of the O-specific polysaccharides (OPS) constituting lipopolysaccharide (LPS) of some Pseudomonas species. The capacity of bacteriophage to adsorb to LPS molecules was tested.

摘要

研究了位于革兰氏阴性菌细胞壁上的潜在噬菌体受体的结构，这些细菌保藏于白俄罗斯微生物收藏馆。通过 1D 和 2D H 和 C NMR 光谱研究，阐明了构成某些假单胞菌属脂多糖（LPS）的 O-特异性多糖（OPS）的结构。测试了噬菌体对 LPS 分子的吸附能力。