Temple G S, Ayling P D, Wilkinson S G
Microbios. 1986;45(183):81-91.
Phage H22 was isolated from sewage using Pseudomonas aeruginosa NCTC 8505 (serotype 0:3) as the host. Although not O-specific, this phage was found to have lipopolysaccharide (LPS) as a receptor. The broad host-range and lack of O-specificity of the phage suggested that its receptor site was in the core region of the LPS. Phage H22 had a Bradley type A structure. It was unaffected by chloroform and diethyl ether, and was stable between pH 5 and 8 and in the temperature range 0 to 60 degrees C. The adsorption rate constant was 14.6 X 10(-9) ml min-1. The phage had a latent period of 43 min, with a rise time of 18 min and a burst size of 6. The adsorption of phage to whole cells and LPS occurred over a broad pH range. Maximum adsorption occurred at 50 degrees C and pH 7.5 in the presence of 0.001 M Ca2+.
噬菌体H22是从污水中分离出来的，使用铜绿假单胞菌NCTC 8505（血清型0:3）作为宿主。虽然该噬菌体不是O特异性的，但发现其以脂多糖（LPS）作为受体。该噬菌体的广泛宿主范围和缺乏O特异性表明其受体位点位于LPS的核心区域。噬菌体H22具有布拉德利A型结构。它不受氯仿和乙醚的影响，在pH 5至8之间以及0至60摄氏度的温度范围内稳定。吸附速率常数为14.6×10^(-9) ml min^(-1)。该噬菌体的潜伏期为43分钟，上升时间为18分钟，裂解量为6。噬菌体对全细胞和LPS的吸附在较宽的pH范围内发生。在0.001 M Ca2+存在下，50摄氏度和pH 7.5时吸附量最大。
