Department of Environmental Engineering, Nanjing Forestry University, Nanjing 210037, China.
Department of Environmental Engineering, Nanjing Forestry University, Nanjing 210037, China; Department of Microbiology, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Bioresour Technol. 2018 Oct;266:176-180. doi: 10.1016/j.biortech.2018.06.079. Epub 2018 Jun 23.
Biogenic nanoparticles are promising materials for their green synthesis method and good performance in stimulation on reduction of environmental contaminants. In this study, Pd(0) nanoparticles (bio-Pd) were generated by Klebsiella oxytoca GS-4-08 in fermentative condition and in-situ improved the azo dye reduction. The bio-Pd was mainly located on cell membrane with a size range of 5-20 nm by TEM and XRD data analyses. Anthraquinone-2-disulfonate (AQS) greatly increased the reduction rate of Pd(II) with a reduction efficiency as high as 96.54 ± 0.23% in 24 h. The quinone respiration theory, glucose metabolism and the biohydrogen pathway were used to explain the enhancement mechanism of the in-situ generated bio-Pd on azo dye reduction. These results indicate that the in-situ generated bio-Pd by K. oxytoca strain is efficient for azo dye reduction without complex preparation processes, which is of great significance for the removal and subsequent safe disposal of hazardous environmental compounds.
生物成因纳米颗粒因其绿色合成方法和在刺激环境污染物还原方面的良好性能而备受关注。在本研究中，通过发酵条件下的氧化葡萄糖酸杆菌 GS-4-08 生成了 Pd(0)纳米颗粒（生物-Pd），并原位提高了偶氮染料的还原性能。通过 TEM 和 XRD 数据分析，生物-Pd 主要位于细胞膜上，尺寸范围为 5-20nm。蒽醌-2-磺酸钠（AQS）极大地提高了 Pd(II)的还原速率，在 24 小时内的还原效率高达 96.54±0.23%。醌呼吸理论、葡萄糖代谢和生物氢途径被用来解释原位生成的生物-Pd 对偶氮染料还原的增强机制。这些结果表明，氧化葡萄糖酸杆菌菌株原位生成的生物-Pd 可有效还原偶氮染料，而无需复杂的制备过程，这对于去除和随后安全处理危险环境化合物具有重要意义。
