Department of Biomedical Engineering and Biological Design Center, Boston University, Boston, MA, USA.
Nat Methods. 2018 Jul;15(7):519-522. doi: 10.1038/s41592-018-0042-y. Epub 2018 Jul 2.
We developed a method in which the NS3 cis-protease from hepatitis C virus can be used as a ligand-inducible connection to control the function and localization of engineered proteins in mammalian cells. To demonstrate the versatility of this approach, we designed drug-sensitive transcription factors and transmembrane signaling proteins, the activities of which can be tightly and reversibly controlled through the use of clinically tested antiviral protease inhibitors.
我们开发了一种方法，利用丙型肝炎病毒的 NS3 内切蛋白酶作为配体诱导连接，以控制工程蛋白在哺乳动物细胞中的功能和定位。为了证明这种方法的多功能性，我们设计了药物敏感型转录因子和跨膜信号蛋白，其活性可以通过使用临床测试的抗病毒蛋白酶抑制剂进行紧密和可逆的控制。
