The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou 325027, China.
The Second School of Medicine, Wenzhou Medical University, Wenzhou 325027, China.
J Diabetes Res. 2018 Jun 5;2018:7504614. doi: 10.1155/2018/7504614. eCollection 2018.
Hyperglycemia is one of the main causes of proliferative diabetic retinopathy (PDR) characterized by thickening of the vascular basement membrane. Laminin alpha 1 (LAMA1) is a primary component of laminin, a major protein constituent of the basement membrane. In this study, we investigated the role of LAMA1 in the development of PDR. Retinal choroidal vascular endothelial cells (RF/6A line) were exposed to glucose at different concentrations (5 mM, 15 mM, 25 mM, and 35 mM) and analyzed for cell growth, migration, proliferation, and adhesion. LAMA1 expression was examined 24 and 48 h following glucose treatment using Western blotting, RT-PCR, and immunofluorescence. The results showed that the proliferation, migration, and adhesion of RF/6A cells were increased by high glucose, whereas LAMA1 expression was slightly higher at 15 mM but decreased at 25 mM and 35 mM glucose compared to control. Thus, the changes in the biological behavior of high glucose-exposed retinal vascular endothelial cells correspond to variations in LAMA1 expression, indicating a possibility for LAMA1 involvement in PDR development. Our findings suggest that LAMA1 may play a role in PDR and, thus, may serve as a potential target for DR diagnosis and/or treatment.
高血糖是增生型糖尿病视网膜病变（PDR）的主要原因之一，其特征是血管基底膜增厚。层粘连蛋白α 1（LAMA1）是层粘连蛋白的主要成分之一，层粘连蛋白是基底膜的主要蛋白成分。在本研究中，我们研究了 LAMA1 在 PDR 发展中的作用。将视网膜脉络膜血管内皮细胞（RF/6A 系）暴露于不同浓度的葡萄糖（5mM、15mM、25mM 和 35mM）中，并分析细胞生长、迁移、增殖和黏附情况。用 Western blot、RT-PCR 和免疫荧光法在葡萄糖处理后 24 和 48 小时检测 LAMA1 表达。结果表明，高葡萄糖促进 RF/6A 细胞的增殖、迁移和黏附，而 LAMA1 的表达在 15mM 时略高，但在 25mM 和 35mM 葡萄糖时与对照相比降低。因此，高葡萄糖暴露的视网膜血管内皮细胞的生物学行为变化与 LAMA1 表达的变化相对应，表明 LAMA1 可能参与 PDR 的发展。我们的研究结果表明，LAMA1 可能在 PDR 中发挥作用，因此可能成为 DR 诊断和/或治疗的潜在靶点。
