Aloni Y, Hay N
CRC Crit Rev Biochem. 1985;18(4):327-83. doi: 10.3109/10409238509086785.
In eukaryotes, an abundant population of promoter-proximal RNA chains have been observed and studied, mainly in whole nuclear RNA, in denovirus type 2, and in SV40. On the basis of these results it has been suggested that a premature termination process resembling attenuation in prokaryotes occurs in eukaryotes. Moreover, these studies have shown that the adenosine analog 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole (DRB) enhances premature termination, but its mode of action is not understood. The determination of the nucleotide sequences of SV40 and other viruses and cellular genes provide means for elucidating the nucleotide sequences involved in the attenuation mechanism. A model has recently been described in which attenuation and mRNA modulation in a feedback control system quantitatively regulate SV40 gene expression. The suggested mechanism described in this model opens up approaches to the investigation of attenuation and mRNA modulation as a possible mechanism whereby eukaryotes may regulate transcription in a variety of different circumstances.
在真核生物中，已观察并研究了大量启动子近端RNA链，主要存在于全核RNA、腺病毒2型和SV40中。基于这些结果，有人提出真核生物中发生了类似于原核生物衰减的过早终止过程。此外，这些研究表明，腺苷类似物5,6 - 二氯 - 1 - β - D - 呋喃核糖基苯并咪唑（DRB）可增强过早终止，但其作用方式尚不清楚。SV40及其他病毒和细胞基因核苷酸序列的测定为阐明参与衰减机制的核苷酸序列提供了手段。最近描述了一个模型，其中反馈控制系统中的衰减和mRNA调节定量调节SV40基因表达。该模型中提出的机制为研究衰减和mRNA调节开辟了途径，这可能是真核生物在各种不同情况下调节转录的一种机制。
