Skare J A, Schrotel K R
Environ Mutagen. 1985;7(4):563-76. doi: 10.1002/em.2860070414.
An alkaline elution assay was used to measure DNA damage in the rat testis after in vivo treatment with chemicals. All of the chemicals reported to induce heritable mutations in a mammalian assay (mouse specific locus test) were positive in the rat alkaline elution assay. Chemicals that are negative or inconclusive in the specific locus test did not cause detectable DNA damage in rat testes. DNA damage was detected by alkaline elution after either intraperitoneal or oral administration of chemical mutagens. Data from these validation studies were used to establish criteria to be used for evaluation of alkaline elution results with materials of unknown potential for genotoxic effects in germinal tissue.
采用碱性洗脱试验来测量经化学物质体内处理后大鼠睾丸中的DNA损伤。所有在哺乳动物试验（小鼠特定位点试验）中报道可诱导遗传性突变的化学物质，在大鼠碱性洗脱试验中均呈阳性。在特定位点试验中呈阴性或结果不确定的化学物质，不会在大鼠睾丸中造成可检测到的DNA损伤。经腹腔或口服化学诱变剂后，通过碱性洗脱检测到了DNA损伤。这些验证研究的数据被用于建立标准，以评估对生殖组织中具有潜在遗传毒性效应的未知物质进行碱性洗脱试验的结果。
