Doerjer G, Buchholz U, Kreuzer K, Oesch F
Institut für Toxikologie, Mainz, Federal Republic of Germany.
Int Arch Occup Environ Health. 1988;60(3):169-74. doi: 10.1007/BF00378693.
The majority of DNA lesions resulting from interactions of carcinogens with DNA are usually either single strand breaks or lesions which are converted to single strand breaks by treatment of DNA with alkaline solutions. A sensitive method of detecting DNA single strand breaks is the alkaline filter elution of DNA. We started to test this method for biomonitoring occupational exposure with sensitive experimental conditions using pH 12.6, where most alkali-labile DNA lesions are converted to single strand breaks. Under our conditions statistically significant differences can be detected between the elution rates of untreated V79 cells and cells treated with [3H]-thymidine 24 h prior to the elution. Statistically significant increases were detected in the elution rates of male smoking automobile mechanics and male smoking painters compared to non-smoking controls. No statistically significant differences were detected in the elution rates of male non-smoking automobile mechanics and male workers with a suspected exposure to halogenated aromatics compared to male controls. No statistically significant differences were observed in the elution rates of female smoking dry-cleaning workers compared to female smoking controls. Our experience showed that the alkaline elution technique can be a valuable tool for monitoring DNA damage in peripheral lymphocytes in man.
致癌物与DNA相互作用产生的大多数DNA损伤通常要么是单链断裂，要么是通过用碱性溶液处理DNA而转化为单链断裂的损伤。检测DNA单链断裂的一种灵敏方法是DNA的碱性滤膜洗脱法。我们开始在pH 12.6这种敏感的实验条件下测试该方法用于职业暴露生物监测，在这种条件下大多数对碱不稳定的DNA损伤会转化为单链断裂。在我们的实验条件下，可检测到未处理的V79细胞与洗脱前24小时用[3H] - 胸腺嘧啶核苷处理的细胞的洗脱率之间存在统计学上的显著差异。与不吸烟的对照组相比，男性吸烟汽车修理工和男性吸烟油漆工的洗脱率有统计学上的显著增加。与男性对照组相比，男性不吸烟汽车修理工和疑似接触卤代芳烃的男性工人的洗脱率未检测到统计学上的显著差异。与女性吸烟对照组相比，女性吸烟干洗工的洗脱率未观察到统计学上的显著差异。我们的经验表明，碱性洗脱技术可以成为监测人体外周淋巴细胞DNA损伤的一种有价值的工具。
