Center for Evolutionary Chemical Biology, Department of Chemistry University of Copenhagen , Universitetsparken 5 , 2100 Copenhagen , Denmark.
ACS Comb Sci. 2018 Aug 13;20(8):492-498. doi: 10.1021/acscombsci.8b00052. Epub 2018 Jul 18.
Monosized beads of polar resins were synthesized for combinatorial chemistry and chemical biology by sustainable microchannel flow synthesis. Regular, biocompatible, and optically encoded beads could be efficiently prepared on large scale and in high yield. In a preparative flow polymerization instrument, taking advantage of a designed T-connector for droplet formation, quality beads were synthesized with accurate size control using a minimal amount of recirculating silicon oil as suspension medium. Bead-size was controlled through shear imposed by the silicon oil flow rate. This process provided 86% yield of ∼500 μm macrobeads beads within a 20 μm size range with no deformities or vacuoles, ideally suited for combinatorial chemistry and protein binding studies. The simple flow equipment consisted of a syringe pump for monomer and initiator delivery, a T-connector, a gear pump for oil recirculation, a long, heated coil of Teflon tubing and a collector syringe. The method was used for preparation of PEGA beads, optically encoded with fluorescent microparticles. The microparticle matrix (MPM) encoded beads were tested in a MPM-decoder showing excellent recognition in bead decoding.
通过可持续的微通道流合成，合成了用于组合化学和化学生物学的单分散性极性树脂珠。可以高效地、大规模地、高产率地制备规则的、生物相容性的、光学编码的珠子。在制备型流动聚合仪器中，利用设计的 T 型连接器进行液滴形成，使用最小量的循环硅油作为悬浮介质，通过硅油压的剪切作用实现精确的粒径控制，从而合成高质量的珠子。该过程可提供 86%的产率，得到 500μm 左右的大珠，粒径范围为 20μm，无变形或空洞，非常适合组合化学和蛋白质结合研究。简单的流路设备包括用于单体和引发剂输送的注射器泵、T 型连接器、用于油循环的齿轮泵、长的、加热的聚四氟乙烯管线圈和收集注射器。该方法用于制备 PEGA 珠，并用荧光微球对其进行光学编码。在 MPM 解码器中对微球矩阵 (MPM) 编码珠进行了测试，显示出在珠解码中的优异识别能力。
