Department of Chemistry, Jadavpur University, 188 Raja S. C. Mallick Road, Kolkata 700032, India.
Department of Chemistry, Mahadevananda Mahavidyalaya, Barrackpore, Kolkata 700120, India.
Int J Biol Macromol. 2018 Oct 15;118(Pt A):1203-1210. doi: 10.1016/j.ijbiomac.2018.06.180. Epub 2018 Jun 30.
The prime motivation of the present study is to explore the effect of reverse micellar confinement on the binding interaction of an anthracene-based probe 9-methyl anthroate with herring-sperm DNA. The structural modification of the genomic DNA from its native B-form to the non-native C-form and subsequently to the condensed Ψ-form as a function of the level of hydration (W, defined as [water] / [surfactant]) of the reverse micellar core is found to reveal a remarkable regulatory role on the stability of the stacking interaction (intercalation) of the probe within the DNA helix; the interaction being progressively stabilized at higher W. Particularly, a close perusal of the dynamical aspects of the interaction is found to be counter-intuitive to the popular notion of the properties of the confined water within the reverse micelles typically approaching bulk-like properties at sufficiently high hydration levels (W > 10).
本研究的主要动机是探索反胶束限制对蒽基探针 9-甲基蒽酸与鲱鱼精子 DNA 结合相互作用的影响。发现基因组 DNA 的结构从天然 B 型到非天然 C 型，再到凝聚的 Ψ 型的修饰，作为反胶束核中水含量（W，定义为[水]/[表面活性剂]）的函数，对探针在 DNA 螺旋内的堆积相互作用（嵌入）的稳定性具有显著的调节作用；随着 W 的增加，相互作用逐渐稳定。特别是，对相互作用动力学方面的仔细研究发现，与反胶束内受限水的性质通常在足够高的水合水平（W>10）接近类似体相的性质的流行观点相矛盾。
