Tanaka Y, Inoi T, Tozawa H, Yamamoto N, Hinuma Y
Int J Cancer. 1985 Nov 15;36(5):549-55. doi: 10.1002/ijc.2910360506.
We have prepared two new mouse monoclonal antibodies (MAbs) named TARM-34 (IgM) and TAG-34 (IgG1), that react with surface antigens of lines of human lymphocytes bearing a human T-cell leukemia virus type-I (HTLV-I). The characters of these antibodies are compared with those of anti-HTLV-1 gp21 MAb (TA-21, IgG1), anti-HTLV-I p19 MAb (GIN-14, IgG1) and human antibodies from patients with adult T-cell leukemia (ATL). An indirect membrane immunofluorescence assay showed that TARM-34, TAG-34 and TA-21 all reacted specifically with cell-surface antigens of HTLV-I-positive T- and B-cell lines and cultured peripheral blood lymphocytes from HTLV-I-infected adults. Radioimmunoassay showed that serum antibodies from the ATL patients interfered with the binding of TA-21 antibody to cells of the HTLV-I-positive T-cell line MT-2, but not with the bindings of TARM-34 and TAG-34 antibodies. TARM-34 and TAG-34 both precipitated a 34-kd glycoprotein (gP34), while TA-21 precipitated gp21 from a lysate of 3H-glucosamine-labelled MT-2 cells. TARM-34 and TAG-34 also precipitated the 34-kd protein from lysates of MT-2 and HUT 102 cells labelled with 125I- or 35S-cysteine. Interestingly, TARM-34 and TAG-34 also precipitated 35-kd protein from a lysate of other HTLV-I-positive cells (F-Taj cell line) derived from an ATL patient. TA-21 precipitated the 21-kd protein from the lysates of 35S-cysteine-labelled HTLV-IMT-2 virions, but TARM-34 and TAG-34 did not precipitate any protein from this lysate. TARM-34 lysed HTLV-I-bearing cells in the presence of rabbit complement. These results indicate that TARM-34 and TAG-34 both recognize a glycoprotein antigen that is expressed on the surface of HTLV-I-infected cells.
我们制备了两种新的小鼠单克隆抗体（MAb），分别命名为TARM - 34（IgM）和TAG - 34（IgG1），它们可与携带I型人类T细胞白血病病毒（HTLV - I）的人类淋巴细胞系的表面抗原发生反应。将这些抗体的特性与抗HTLV - 1 gp21单克隆抗体（TA - 21，IgG1）、抗HTLV - I p19单克隆抗体（GIN - 14，IgG1）以及来自成人T细胞白血病（ATL）患者的人类抗体的特性进行了比较。间接膜免疫荧光分析表明，TARM - 34、TAG - 34和TA - 21均与HTLV - I阳性T细胞和B细胞系的细胞表面抗原以及来自HTLV - I感染成人的培养外周血淋巴细胞发生特异性反应。放射免疫分析表明，ATL患者的血清抗体干扰TA - 21抗体与HTLV - I阳性T细胞系MT - 2细胞的结合，但不干扰TARM - 34和TAG - 34抗体的结合。TARM - 34和TAG - 34均沉淀出一种34kd的糖蛋白（gP34），而TA - 21从3H - 葡糖胺标记的MT - 2细胞裂解物中沉淀出gp21。TARM - 34和TAG - 34还从用125I或35S - 半胱氨酸标记的MT - 2和HUT 102细胞裂解物中沉淀出34kd的蛋白质。有趣的是，TARM - 34和TAG - 34还从一名ATL患者来源的其他HTLV - I阳性细胞（F - Taj细胞系）的裂解物中沉淀出35kd的蛋白质。TA - 21从用35S - 半胱氨酸标记的HTLV - I MT - 2病毒粒子裂解物中沉淀出21kd的蛋白质，但TARM - 34和TAG - 34从该裂解物中未沉淀出任何蛋白质。TARM - 34在兔补体存在下可裂解携带HTLV - I的细胞。这些结果表明，TARM - 34和TAG - 34均识别一种在HTLV - I感染细胞表面表达的糖蛋白抗原。
