Deng Junfang, Zhou Bo, Lin Bingyi, Geng Lei, Shen Tian, Shu Rong, Xie Haiyang, Zheng Shusen
Department of Hepatobiliary Pancreatic Surgery, The First Affiliated Hospital, School of Medicine, Zhejiang University, Key Lab of Organ Transplantation of Zhejiang Province, Key Lab of Combined Multi-Organ Transplantation, Ministry of Public Health, Hangzhou, China.
Department of Hepatobiliary Pancreatic Surgery, The First Affiliated Hospital, School of Medicine, Zhejiang University, Key Lab of Organ Transplantation of Zhejiang Province, Key Lab of Combined Multi-Organ Transplantation, Ministry of Public Health, Hangzhou, China
Ann Clin Lab Sci. 2018 May;48(3):328-332.
CD16 monocytes have recently shown the ability to inhibit the proliferation of CD4CD25Foxp3 T cells (Tregs). The inhibitory role of Tregs in acute rejection has been described in patients with liver transplantation. However, the role of CD16 monocytes in the development of allograft rejection after liver transplantation remains unknown.
Forty-five liver transplant recipients, including 25 acute rejection diagnosed by liver biopsy and clinical symptoms and 20 stable allograft liver function recipients, were collected from January 2007 to September 2015 at our hospitals. To assay the frequencies of CD16 monocytes and Tregs in blood samples, flow cytometry was performed.
Compared with the stable allograft liver function recipients, a significant increase in CD16 monocytes (19.45±5.25% vs 7.17±1.69%, <0.001) and decreased Tregs (1.74±0.59% vs 5.53±2.18%, <0.001) was observed among recipients with acute rejection. CD16 monocytes were positively correlated with RAI (rejection activity index) (R=0.84, <0.001), but negatively correlated with the frequency of Tregs (R=0.86, <0.001).
Based on our data, we concluded that CD16 monocytes might be responsible for the development of acute allograft rejection after liver transplantation, which may be associated with inhibition of Treg cells.
CD16单核细胞最近显示出抑制CD4CD25Foxp3 T细胞（调节性T细胞）增殖的能力。调节性T细胞在肝移植患者急性排斥反应中的抑制作用已有描述。然而，CD16单核细胞在肝移植后同种异体移植排斥反应发生过程中的作用尚不清楚。
2007年1月至2015年9月期间，从我们医院收集了45例肝移植受者，其中25例经肝活检和临床症状诊断为急性排斥反应，20例移植肝功能稳定的受者。采用流式细胞术检测血样中CD16单核细胞和调节性T细胞的频率。
与移植肝功能稳定的受者相比，急性排斥反应受者中CD16单核细胞显著增加（19.45±5.25%对7.17±1.69%，<0.001），调节性T细胞减少（1.74±0.59%对5.53±2.18%，<0.001）。CD16单核细胞与排斥活性指数（RAI）呈正相关（R=0.84，<0.001），但与调节性T细胞频率呈负相关（R=0.86，<0.001）。
根据我们的数据，我们得出结论，CD16单核细胞可能是肝移植后急性同种异体移植排斥反应发生的原因，这可能与调节性T细胞的抑制有关。
