Liao Ching-Lung, Chu Yung Lin, Lin Hui-Yi, Chen Cheng-Yen, Hsu Ming-Jie, Liu Kuo-Ching, Lai Kuang-Chi, Huang An-Cheng, Chung Jing-Gung
College of Chinese Medicine, School of Post-Baccalaureate Chinese Medicine, China Medical University, Taichung, Taiwan, R.O.C.
International Master's Degree Program in Food Science, International College, National Pingtung University of Science and Technology, Pingtung, Taiwan, R.O.C.
Anticancer Res. 2018 Jul;38(7):3989-3997. doi: 10.21873/anticanres.12686.
BACKGROUND/AIM: Bisdemethoxycurcumin (BDMC) exhibits biological activities including anticancer and anti-metastasis in human cancer cell lines, but there is no available information to show whether BDMC suppresses cell migration and invasion of human cervical cancer cells.
Wound-healing, migration, invasion, zymography, and western blotting assays were used to investigate the effects of BDMC on HeLa cells in vitro.
BDMC reduced the total viable cell number in a dose-dependent manner. The wound-healing assay show BDMC suppressed the movement of HeLa cells. Furthermore, the trans-well chamber assays showed that BDMC suppressed the cell migration and invasion. Gelatin zymograph assay showed that BDMC did not inhibit matrix metalloproteinase-2 (MMP-2) and -9 activities in vitro. However, western blotting assay showed that BDMC significantly reduced protein levels of growth factor receptor-bound protein 2 (GRB2), Ras homolog gene family, member A (Rho A), urokinase-type plasminogen activator (uPA), RAS, MMP-2, and N-cadherin but increased those of phosphor-extracellular-signal related kinase (p-ERK1/2), E-cadherin and nuclear factor-ĸB (NF-ĸB) in HeLa cells. Confocal laser microscopy assay was used to further confirm BDMC increased NF-ĸB when compared to controls.
BDMC may have potential as a novel anti-metastasis agent for the treatment of human cervical cancer.
背景/目的：双去甲氧基姜黄素（BDMC）在人癌细胞系中表现出包括抗癌和抗转移在内的生物学活性，但尚无信息表明BDMC是否能抑制人宫颈癌细胞的迁移和侵袭。
采用伤口愈合、迁移、侵袭、酶谱分析和蛋白质印迹分析等方法研究BDMC对体外培养的HeLa细胞的影响。
BDMC以剂量依赖的方式降低了总活细胞数。伤口愈合试验表明BDMC抑制了HeLa细胞的移动。此外，Transwell小室试验表明BDMC抑制了细胞迁移和侵袭。明胶酶谱分析表明BDMC在体外不抑制基质金属蛋白酶-2（MMP-2）和-9的活性。然而，蛋白质印迹分析表明BDMC显著降低了HeLa细胞中生长因子受体结合蛋白2（GRB2）、Ras同源基因家族成员A（Rho A）、尿激酶型纤溶酶原激活剂（uPA）、RAS、MMP-2和N-钙黏蛋白的蛋白水平，但增加了磷酸化细胞外信号调节激酶（p-ERK1/2）、E-钙黏蛋白和核因子-κB（NF-κB）的蛋白水平。共聚焦激光显微镜分析进一步证实与对照组相比BDMC增加了NF-κB的表达。
BDMC可能具有作为新型抗转移药物治疗人宫颈癌的潜力。
