Department of Obstetrics and Gynecology, Minhang Hospital, Fudan University, Shanghai, China.
Department of Medicinal Chemistry, School of Pharmacy, Fudan University, Shanghai, China.
Skin Res Technol. 2024 Sep;30(9):e70042. doi: 10.1111/srt.70042.
Value analysis of a small-molecule fluorescent probe for methylation detection in different cervical lesions.
(1) The grayscale values of distinct lesion tissues were remarkably distinct among the four groups (p < 0.05). The comparison of the grayscale value between the two groups showed that the CA group noticeably exceeded the LSIL and cervicitis groups, and the HSIL group was apparently higher than the LSIL and cervicitis groups (p < 0.05); (2) The mean grayscale values of the enrolled subjects were calculated with 55.21 as the midline, with >55.21 as positive and ≤55.21 as negative.
The results showed that the positive rate of the cervicitis group was 0.00%, the LSIL group 67.74%, the HSIL group 83.33%, and the CA group 100.00%. The results among the four groups were notably distinct (p < 0.05); (3) The comparison among DAPI, probe, bright, and merged images of cervicitis, LSIL, HSIL, and CA indicated that different cervical lesions were with quite various stains.
The grayscale value, positive rate, and stained picture of distinct cervical lesions were remarkably different. The small-molecule fluorescent probe has a good value in differentiating cervical lesions and can be considered for popularization and application.
一种用于检测不同宫颈病变中甲基化的小分子荧光探针的价值分析。
（1）四组间不同病变组织的灰度值差异有统计学意义（p<0.05）。两组间灰度值比较显示，CA 组明显高于 LSIL 组和宫颈炎组，HSIL 组明显高于 LSIL 组和宫颈炎组（p<0.05）；（2）计算纳入对象的平均灰度值，以 55.21 为中线，>55.21 为阳性，≤55.21 为阴性。
宫颈炎组阳性率为 0.00%，LSIL 组为 67.74%，HSIL 组为 83.33%，CA 组为 100.00%。四组间结果差异有统计学意义（p<0.05）；（3）DAPI、探针、亮场和合并图像比较宫颈炎、LSIL、HSIL 和 CA 表明，不同的宫颈病变具有不同的染色。
不同宫颈病变的灰度值、阳性率和染色图片差异显著。该小分子荧光探针在鉴别宫颈病变方面具有较好的应用价值，值得推广应用。
