腹水浸润γδ T细胞在过继性免疫治疗中的潜在应用
Possible Application of Ascites-infiltrating Gamma-delta T Cells for Adoptive Immunotherapy.
作者信息
Abe Yuki, Kobayashi Hirohito, Akizawa Yoshika, Ishitani Ken, Hashimoto Kazunori, Matsui Hideo
机构信息
Department of Obstetrics and Gynecology, Tokyo Women's Medical University, Tokyo, Japan.
Department of Transfusion Medicine and Cell Processing, Tokyo Women's Medical University, Tokyo, Japan.
出版信息
Anticancer Res. 2018 Jul;38(7):4327-4331. doi: 10.21873/anticanres.12732.
BACKGROUND/AIM: Malignant ascites contain many tumour-infiltrating lymphocytes. γδ T cells with antitumour activity have attracted attention as effector cells in cancer immunotherapy. Vδ T cells were cultured from peripheral blood mononuclear cells (PBMCs) and ascites-infiltrating lymphocytes (AILs) to compare the differences in response to 2-methyl-3-butenyl-1-pyrophosphate (2M3B1-PP) and zoledronate (Zol) as antigens in vitro.
MATERIALS AND METHODS
To expand Vδ T cells from PBMCs and AILs from 29 patients with cancer, these cells were cultured and subjected to analysis.
RESULTS
The proliferation rate of Vδ T cells was higher in both PBMCs and AILs when cultured with Zol than with 2M3B1-PP. Although Vδ T cells show a higher rate of expansion in AILs compared to PBMCs, the number of mixed tumour cells in ascites was decreased when cultured with Zol.
CONCLUSION
Vδ T cells in AILs are cytotoxic to tumour cells in ascites and may be considered in adoptive immunotherapy.
背景/目的：恶性腹水中含有许多肿瘤浸润淋巴细胞。具有抗肿瘤活性的γδ T细胞作为癌症免疫治疗中的效应细胞受到关注。从外周血单核细胞（PBMC）和腹水浸润淋巴细胞（AIL）中培养Vδ T细胞，以比较体外对作为抗原的2-甲基-3-丁烯基-1-焦磷酸（2M3B1-PP）和唑来膦酸（Zol）的反应差异。
材料与方法
为了从29例癌症患者的PBMC和AIL中扩增Vδ T细胞，对这些细胞进行培养并进行分析。
结果
与2M3B1-PP相比，用Zol培养时，PBMC和AIL中Vδ T细胞的增殖率更高。尽管与PBMC相比，AIL中Vδ T细胞的扩增率更高，但用Zol培养时，腹水中混合肿瘤细胞的数量减少。
结论
AIL中的Vδ T细胞对腹水中的肿瘤细胞具有细胞毒性，可考虑用于过继性免疫治疗。
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