Li Yuan, Tian Jing, Guo Zi-Jian, Zhang Zhu-Bo, Xiao Chang-Yan, Wang Xiao-Chun
Tianjin Key Laboratory of Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Science, Tianjin 300192, China.
Department of Inner Medicine, Shandong Cancer Hospital and Institute, Jinan 250117, Shandong Province, China.
J Cancer Res Ther. 2018 Jun;14(Supplement):S295-S298. doi: 10.4103/0973-1482.235344.
To explore the expression of microRNA-106b (miRNA-106b) in nonsmall cell lung cancer (NSCLC).
miRNAs are short regulatory RNAs that negatively modulate gene expression at the posttranscriptional level, and are deeply involved in the pathogenesis of several types of cancer. miRNA-106b has been shown to play an oncogenic role in tumor progression. The expression of miRNA-106b is detected in this study.
Quantitative reverse transcription polymerase chain reaction and Northern blotting were used to detect the expression level of miRNA-106b in 200 NSCLC samples.
All statistical analyses were performed using SPSS 16.0 software. Results were statistically evaluated using the Kruskal-Wallis test and Mann-Whitney U-test. Survival curves were estimated by the Kaplan-Meier method and P < 0.05 was considered to be statistically significant.
miRNA-106b expression is increased in NSCLC tissues. Statistical analysis showed that overexpression of miRNA-106b was strongly associated with lymph node metastasis, stage of tumor node metastasis classification, and poor prognosis. Moreover, there was a significant difference in the miRNA-106b expression levels between smoking and nonsmoking patients. Multivariate Cox regression analysis showed that miRNA-106b was an independent prognostic factor for NSCLC patients.
These data suggest that aberrantly expressed miRNA-106b may contribute to the development of NSCLC.
探讨微小RNA-106b（miRNA-106b）在非小细胞肺癌（NSCLC）中的表达情况。
微小RNA是一类短链调节性RNA，可在转录后水平负向调节基因表达，并深度参与多种癌症的发病机制。已有研究表明miRNA-106b在肿瘤进展中发挥致癌作用。本研究检测miRNA-106b的表达情况。
采用定量逆转录聚合酶链反应和Northern印迹法检测200例NSCLC样本中miRNA-106b的表达水平。
所有统计分析均使用SPSS 16.0软件进行。结果采用Kruskal-Wallis检验和Mann-Whitney U检验进行统计学评估。生存曲线采用Kaplan-Meier法估计，P<0.05被认为具有统计学意义。
NSCLC组织中miRNA-106b表达升高。统计分析表明，miRNA-106b的过表达与淋巴结转移、肿瘤淋巴结转移分类分期及预后不良密切相关。此外，吸烟患者与非吸烟患者之间miRNA-106b表达水平存在显著差异。多因素Cox回归分析显示，miRNA-106b是NSCLC患者的独立预后因素。
这些数据表明，异常表达的miRNA-106b可能促进NSCLC的发生发展。
