Chung Yuan-Chiang, Chiang Hua-Che, Chang Hsiang, Lin Chih-Cheng, Lo Li-Tsai, Wang Ai-Yih, Chou Kuo-Feng, Hsu Chih-Ping
Department of Surgery, Cheng Ching Hospital, Chung-Kang Branch, Taichung, Taiwan, China.
Department of Colorectal Surgery, China Medical University Hospital, Taichung, China.
J Cancer Res Ther. 2018 Jun;14(Supplement):S388-S393. doi: 10.4103/0973-1482.176170.
Proanthocyanidin-rich longan flower extract (LFP) has been previously shown to inhibit the proliferation and anchorage-independent growth in soft agar of two colorectal carcinoma (CRC) cells in vitro. In this report, we further examined the effects of LFP in a CRC spheroid model.
A liquid-overlay assay employing HT-29 spheroids was used to evaluate the effects of LFP on cancer cell tumorigenesis, viability, and apoptosis. Associated effects on signaling path ways (epidermal growth factor receptor [EGFR], Akt) and apoptotic regulators were measured using Western blot.
Treatment with LFP up to 200 μg/ml inhibited tumor growth in a dose-dependent manner and induced prominent apoptosis as measured by annexin V staining. Cells treated with LFP showed decreased EGFR and Akt phosphorylation with decreased expression of B-cell lymphoma 2.
The ability of LFP to induce apoptosis in CRC spheroids warrants further investigation of its composition and identification of tumor-active components.
富含原花青素的龙眼花提取物（LFP）先前已被证明在体外可抑制两种结肠直肠癌（CRC）细胞在软琼脂中的增殖和非锚定依赖性生长。在本报告中，我们进一步研究了LFP在CRC球体模型中的作用。
采用HT-29球体的液体覆盖试验来评估LFP对癌细胞肿瘤发生、活力和凋亡的影响。使用蛋白质印迹法检测对信号通路（表皮生长因子受体[EGFR]、Akt）和凋亡调节因子的相关影响。
高达200μg/ml的LFP处理以剂量依赖性方式抑制肿瘤生长，并通过膜联蛋白V染色检测到显著的细胞凋亡。用LFP处理的细胞显示EGFR和Akt磷酸化降低，同时B细胞淋巴瘤2的表达降低。
LFP诱导CRC球体细胞凋亡的能力值得进一步研究其成分并鉴定肿瘤活性成分。
