Wu Yong-Yang, Liu Xin-Yu, Zhuo De-Xiang, Huang Huai-Bin, Zhang Fa-Biao, Liao Shang-Fan
Department of Urology, Affiliated Sanming First Hospital, Fujian Medical University, Sanming, Fujian 365100, China.
State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University and Collaborative Innovation Center for Biotherapy, Chengdu, Sichuan 610041, China.
Cancer Manag Res. 2018 Jun 22;10:1647-1655. doi: 10.2147/CMAR.S166390. eCollection 2018.
The aim of this study was to investigate whether the expression of the ligand-gated Ca channel transient receptor potential vanilloid type-1 (TRPV1) in primary human renal cell carcinoma (RCC) is associated with clinicopathological features.
Fresh and frozen primary tumor and normal peritumoral kidney tissues from 127 patients diagnosed with RCC were analyzed for TRPV1 expression by quantitative reverse transcription polymerase chain reaction (RT-PCR), Western blotting and immunohistochemistry.
Quantitative RT-PCR revealed that TRPV1 was decreased 3.20-fold in RCC tissue vs normal peritumoral kidney tissue (=0.012). Significantly different TRPV1 mRNA expression was detected in RCC tissues of different Fuhrman grades and histopathological subtypes (F=4.282, =0.015 and F=5.205, =0.014, respectively). Decreased TRPV1 expression was correlated with RCC histopathological subtype (R=-0.554, =0.003) and Fuhrman grade (R=-0.525, =0.006). Western blot analysis of TRPV1 protein expression showed similar results. Immunohistochemical analysis showed strong expression of TRPV1 in kidney tubules but demonstrated weak or no immunostaining in RCC tissues.
TRPV1 expression was decreased in RCC, which was significantly associated with tumor Fuhrman grades and histopathological subtypes. It seems to suggest that TRPV1 expression may be a valuable tool to predict the extent of RCC progression.
本研究旨在探讨配体门控钙通道瞬时受体电位香草酸受体1型（TRPV1）在原发性人类肾细胞癌（RCC）中的表达是否与临床病理特征相关。
对127例经诊断患有RCC的患者的新鲜及冷冻原发性肿瘤组织和肿瘤周围正常肾组织进行分析，采用定量逆转录聚合酶链反应（RT-PCR）、蛋白质免疫印迹法和免疫组织化学法检测TRPV1的表达。
定量RT-PCR显示，与肿瘤周围正常肾组织相比，RCC组织中TRPV1表达降低了3.20倍（P = 0.012）。在不同Fuhrman分级和组织病理学亚型的RCC组织中检测到TRPV1 mRNA表达存在显著差异（F分别为4.282，P = 0.015和F为5.205，P = 0.014）。TRPV1表达降低与RCC组织病理学亚型（R = -0.554，P = 0.003）和Fuhrman分级（R = -0.525，P = 0.006）相关。TRPV1蛋白表达的蛋白质免疫印迹分析显示了相似的结果。免疫组织化学分析显示TRPV1在肾小管中强表达，但在RCC组织中免疫染色较弱或无免疫染色。
RCC中TRPV1表达降低，这与肿瘤Fuhrman分级和组织病理学亚型显著相关。这似乎表明TRPV1表达可能是预测RCC进展程度的一个有价值的工具。
