Raymond Eder María L, Conti Francisco, Rosa Alberto L
Laboratorio de Genética y Biología Molecular, IRNASUS-CONICET, Facultad de Ciencias Químicas, Universidad Católica de Córdoba, Córdoba, Argentina.
Front Microbiol. 2018 Jun 19;9:1320. doi: 10.3389/fmicb.2018.01320. eCollection 2018.
Yeast communities associated with L. ecosystems have been widely characterized. Less is known, however, about yeast communities present in grapes and fermenting musts from non- ecosystems. Moreover, there are no comparative studies concerning yeast communities in grapes from L. and non- species in vineyards from a shared . In this work, we have used a culture-dependent strategy, phenotypic analyses, and molecular genotyping, to study the most representative yeast species present in spontaneously fermenting musts of grapes harvested from neighboring L. (cv. Malbec) and L. (cv. Isabella) vineyards. Phenotypic analyses of HS production, ethanol tolerance and carbon utilization, on randomly selected strains of each and strains, as well as microsatellite genotyping of isolates from each the Malbec and Isabella grape musts, suggest that L. and L. ecosystems could harbor different yeast strain populations. Thus, microbial communities in exotic species may offer opportunities to look for unique yeast strains that could not be present in conventional L. ecosystems.
与L.生态系统相关的酵母群落已得到广泛表征。然而，对于非L.生态系统中葡萄和发酵葡萄汁中存在的酵母群落了解较少。此外，没有关于来自共享地区的L.葡萄园和非L.葡萄园葡萄中酵母群落的比较研究。在这项工作中，我们采用了依赖培养的策略、表型分析和分子基因分型，来研究从相邻的L.（马尔贝克品种）和L.（伊莎贝拉品种）葡萄园收获的葡萄自然发酵葡萄汁中最具代表性的酵母种类。对每个品种随机选择的菌株以及来自马尔贝克和伊莎贝拉葡萄汁的菌株进行的硫化氢产生、乙醇耐受性和碳利用的表型分析，以及微卫星基因分型表明，L.和L.生态系统可能含有不同的酵母菌株群体。因此，外来品种中的微生物群落可能为寻找传统L.生态系统中不存在的独特酵母菌株提供机会。
