嗜碱假单胞菌中磷酸核酮糖激酶基因的染色体和质粒定位。
Chromosomal and plasmid locations for phosphoribulokinase genes in Alcaligenes eutrophus.
作者信息
Klintworth R, Husemann M, Salnikow J, Bowien B
出版信息
J Bacteriol. 1985 Nov;164(2):954-6. doi: 10.1128/jb.164.2.954-956.1985.
Abstract
Genes coding for phosphoribulokinase (PRK), a key enzyme of the Calvin cycle, were localized in the genome of the chemoautotroph Alcaligenes eutrophus. The NH2-terminal sequence of the PRK subunit was determined. With a synthetic oligodeoxynucleotide probe complementary to a portion of this sequence, hybridization analysis revealed PRK genes to be located on both the chromosome and the megaplasmid pHG1 of A. eutrophus H16.
摘要
编码卡尔文循环关键酶磷酸核酮糖激酶（PRK）的基因定位于化能自养菌嗜碱假单胞菌的基因组中。测定了PRK亚基的氨基末端序列。用与该序列一部分互补的合成寡脱氧核苷酸探针进行杂交分析，结果显示PRK基因位于嗜碱假单胞菌H16的染色体和大质粒pHG1上。
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