Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture, Northeast Agricultural University, 600 Changjiang Road, Harbin, 150030, China.
College of Horticulture and Landscape Architecture, Northeast Agricultural University, 600 Changjiang Road, Harbin, 150030, China.
Mol Genet Genomics. 2018 Dec;293(6):1393-1405. doi: 10.1007/s00438-018-1467-9. Epub 2018 Jul 3.
Dwarf mutations have played vital roles in elucidating the regulatory molecular mechanisms of plant height. In this study, we identified a mutant named Csdw, whose mutagenesis was induced by ethyl methyl sulfonate in cucumber, and this mutant exhibited a dwarf phenotype with a reduced internode length because of the reduction of cell division in the main stem. The dwarf phenotype of Csdw could be partially rescued through GA application, and endogenous GA levels from the stem of Csdw decreased distinctly. Genetic analysis showed that Csdw was attributed to a recessive gene. The MutMap and Kompetitive Allele Specific PCR genotyping results revealed that Csa3G872760 (CsCLAVATA1), encoding a CLAVATA1-type receptor-like kinase, was a putative candidate gene for dwarf mutation in cucumber. The expression of CsCLAVATA1 in the stem of Csdw was lower than that of wild-type plants. Therefore, CsCLAVATA1 could regulate the dwarf phenotype in cucumber.
矮化突变在阐明植物高度的调控分子机制方面发挥了重要作用。本研究中，我们鉴定了一个名为 Csdw 的突变体，其诱变剂是在黄瓜中使用乙基甲磺酸诱导的，该突变体表现出矮化表型，主茎的细胞分裂减少导致节间长度缩短。Csdw 的矮化表型可以通过 GA 处理部分挽救，并且 Csdw 茎中的内源 GA 水平明显降低。遗传分析表明，Csdw 是一个隐性基因。MutMap 和 Kompetitive Allele Specific PCR 基因分型结果表明，编码 CLAVATA1 型受体样激酶的 Csa3G872760（CsCLAVATA1）是黄瓜矮化突变的一个潜在候选基因。Csdw 茎中的 CsCLAVATA1 表达低于野生型植株。因此，CsCLAVATA1 可以调控黄瓜的矮化表型。
