在玫烟色棒束孢分生孢子和小菌核发育过程中通过 Pbs2 适应应激。
Adaption to stress via Pbs2 during Metarhizium rileyi conidia and microsclerotia development.
机构信息
Chongqing Engineering Research Center for Fungal Insecticide, School of Life Science, Chongqing University, Chongqing, 400030, People's Republic of China.
College of Preclinical Medicine, Southwest Medical University, Luzhou, 646000, People's Republic of China.
出版信息
World J Microbiol Biotechnol. 2018 Jul 3;34(8):107. doi: 10.1007/s11274-018-2475-y.
The high osmolarity glycerol (HOG) pathway plays important role in Metarhizium rileyi microsclerotia (MS) development. To investigate how M. rileyi transduce growth stress and regulate MS development via mitogen-activated protein kinase kinase (MAPKK) Pbs2, phenotypic characterization of the yeast Pbs2 homolog were performed. Expression of pbs2 peaked when MS formation occurred day 3 in liquid amended medium. Compared with wild-type and complemented strains, deletion mutant of pbs2 (Δpbs2) delayed dimorphic switch and vegetative growth, displayed sensitivities to various stress, and significantly reduced conidial (98%) and MS (40%) yields. Furthermore, transcription analysis showed that other genes of HOG signaling pathway were down-regulated in Δpbs2 mutants. Insect bioassays revealed that Δpbs2 mutants had decreased virulence levels in topical (24%) and injection (53%) bioassays. This study confirmed that Pbs2 play important roles in colony morphology, conidiation, stresses response and MS development in M. rileyi.
高渗透压甘油（HOG）途径在玫烟色棒束孢微菌核（MS）发育中发挥重要作用。为了研究玫烟色棒束孢如何通过丝裂原活化蛋白激酶激酶（MAPKK）Pbs2 传递生长胁迫并调节 MS 发育，对酵母 Pbs2 同源物的表型特征进行了研究。在液体改良培养基中，当 MS 形成第 3 天时，pbs2 的表达达到峰值。与野生型和互补菌株相比，pbs2 缺失突变体（Δpbs2）延迟了二态转换和营养生长，对各种应激表现出敏感性，并显著降低了分生孢子（98%）和 MS（40%）的产量。此外，转录分析表明，HOG 信号通路的其他基因在Δpbs2 突变体中下调。昆虫生物测定表明，Δpbs2 突变体在局部（24%）和注射（53%）生物测定中的毒力水平降低。本研究证实 Pbs2 在玫烟色棒束孢的菌落形态、产孢、应激反应和 MS 发育中发挥重要作用。
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