Beltsville Human Nutrition Research Center, USDA-ARS, Beltsville, MD, USA.
Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD, USA.
J Sci Food Agric. 2018 Dec;98(15):5572-5580. doi: 10.1002/jsfa.9235. Epub 2018 Sep 17.
Soybean is one of the best sources of plant protein. Development of improved soybean cultivars through classical breeding and new biotech approaches is important to meet the growing global demand for soybeans. There is a critical need to investigate changes in protein content and profiles to ensure the safety and nutritional quality of new soybean varieties and their food products. A proteomics study begins with an optimal combination of extraction, separation and detection approaches. This review attempts to provide a summary of current updates in the methodologies used for extraction, separation and detection of protein from soybean, the basic foundations for good proteomic research. This information can be effectively used to investigate modifications in protein content and profiles in new varieties of soybeans and other crops. © 2018 Society of Chemical Industry.
大豆是植物蛋白的最佳来源之一。通过经典的育种和新的生物技术方法开发改良的大豆品种对于满足全球对大豆日益增长的需求非常重要。因此，有必要研究蛋白质含量和图谱的变化，以确保新型大豆品种及其食品的安全性和营养价值。蛋白质组学研究始于最佳的提取、分离和检测方法的组合。本文综述了从大豆中提取、分离和检测蛋白质的方法的最新进展，这些方法是蛋白质组学研究的基础。这些信息可以有效地用于研究新型大豆和其他作物品种中蛋白质含量和图谱的变化。 © 2018 英国化学学会
