Roguska M A, Gudas L J
J Biol Chem. 1985 Nov 15;260(26):13893-6.
Monolayer cultures of F9 teratocarcinoma stem cells and P19 stem cells differentiate into endoderm, and fibroblast-like cells, respectively, when treated with retinoic acid. We demonstrate that this differentiation is associated with a large increase (greater than 40-fold) in the activity of an enzyme, prolyl-4-hydroxylase, involved in the posttranslational modification of collagens. This large increase in prolyl-4-hydroxylase activity occurs between 42 and 72 h after retinoic acid addition, and is associated with an increased amount of immunoprecipitable prolyl hydroxylase enzyme. This enzyme should be a useful marker for certain differentiated cell types produced during differentiation of teratocarcinoma stem cell lines.
当用视黄酸处理时，F9畸胎瘤干细胞和P19干细胞的单层培养物分别分化为内胚层和成纤维细胞样细胞。我们证明，这种分化与一种参与胶原蛋白翻译后修饰的酶——脯氨酰-4-羟化酶的活性大幅增加（超过40倍）有关。脯氨酰-4-羟化酶活性的这种大幅增加发生在添加视黄酸后的42至72小时之间，并且与可免疫沉淀的脯氨酰羟化酶的量增加有关。这种酶应该是畸胎瘤干细胞系分化过程中产生的某些分化细胞类型的有用标志物。
