Muramatsu H, Muramatsu T
FEBS Lett. 1983 Nov 14;163(2):181-4. doi: 10.1016/0014-5793(83)80814-x.
Teratocarcinoma stem cell F9 expressed a potent fucosyltransferase activity acting on asialofetuin. A majority of the product was susceptible to alpha-L-fucosidase I from almond emulsin, indicating that the linkage formed was mainly Fuc alpha 1 leads to 3GlcNAc. The specific activity of the transferase decreased when the stem cells were induced to differentiate into parietal endoderm cells by retinoic acid and dibutyryl cyclic AMP. Furthermore, PYS-2 cell, a parietal endoderm cell line virtually lacked the transferase. The change in the fucosyltransferase activity could be correlated with cell surface changes occurring during differentiation.
畸胎癌细胞F9表现出对脱唾液酸胎球蛋白具有活性的岩藻糖基转移酶。大部分产物对来自杏仁乳化酶的α-L-岩藻糖苷酶I敏感，表明形成的连接主要是Fucα1→3GlcNAc。当干细胞通过视黄酸和二丁酰环磷酸腺苷诱导分化为壁内胚层细胞时，转移酶的比活性降低。此外，壁内胚层细胞系PYS-2实际上缺乏该转移酶。岩藻糖基转移酶活性的变化可能与分化过程中发生的细胞表面变化相关。
