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黑腹果蝇的D1蛋白。纯化及与AT-DNA的结合特性

D1 protein of Drosophila melanogaster. Purification and AT-DNA binding properties.

作者信息

Levinger L F

出版信息

J Biol Chem. 1985 Nov 15;260(26):14311-8.

PMID:2997203
Abstract

D1 protein of Drosophila melanogaster is a sequence-specific DNA-binding protein which recognizes AT-rich DNA sequences. AT-rich DNA sequences in eukaryotic organisms are distributed in two characteristic ways: flanking transcriptional units and in constitutive heterochromatin. D1 could play a role in regulation of gene expression and in geographical localization of DNA sequences within the nucleus. D1 has been partially purified by ion exchange chromatography. DNA-binding activity was investigated by nucleoprotein gel electrophoresis, using end-labeled restriction fragments varying in AT sequence content. D1 binds most tightly to the satellite sequence -AATAT-, with intermediate strength to the complex satellite (359-base pair repeat) and another AT-rich (68% AT) mixed sequence DNA, and least to the simple satellite sequence -AAGAG-.

摘要

黑腹果蝇的D1蛋白是一种序列特异性DNA结合蛋白，可识别富含AT的DNA序列。真核生物中富含AT的DNA序列以两种特征性方式分布：转录单位侧翼和组成型异染色质中。D1可能在基因表达调控和细胞核内DNA序列的地理定位中发挥作用。D1已通过离子交换色谱法部分纯化。使用AT序列含量不同的末端标记限制性片段，通过核蛋白凝胶电泳研究DNA结合活性。D1与卫星序列-AATAT-结合最紧密，与复杂卫星序列（359碱基对重复）和另一个富含AT（68%AT）的混合序列DNA结合强度中等，与简单卫星序列-AAGAG-结合最弱。

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引用本文的文献

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