Bernardi Lisiane, Souza Bárbara Capitanio de, Sonda Nicole Canalli, Visioli Fernanda, Rados Pantelis Varvaki, Lamers Marcelo Lazzaron
Basic Research Center, Dentistry School, UFRGS - Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
Department of Oral Pathology, Dentistry School, UFRGS - Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
Braz Dent J. 2018 May-Jun;29(3):309-315. doi: 10.1590/0103-644020181868.
The aim of this study was to investigate salivary levels of TGFβ1 and proliferation/ maturation of epithelial mucosa cells in diabetic and hypertensive patients.
in this cross-sectional study, whole stimulated saliva and oral mucosa exfoliative cytology specimens were collected from 39 patients that were healthy (control, n=10) or presented history of arterial hypertension (HAS, n=9), diabetes mellitus (DM, n=10) or both (DM+HAS, n=10). Salivary flow rate (SFR), TGFβ1 level in saliva, AgNORs and the epithelial maturation were evaluated. Non-parametric Kruskal-Wallis test, followed by Dunn's multiple comparison post-test and the Spearman test correlation analysis were used. SFR showed a significant decreased in DM and DM+HAS (0.47±0.11 and 0.64±0.43 mL/min) when compared to control (1.4±0.38 mL/min). DM+HAS presented the highest value of TGFβ1 concentration (24.72±5.89 pg/mL). It was observed a positive correlation between TGFβ1 and glycaemia (R=0.6371; p<0.001) and a negative correlation between TGFβ1 and saliva (R=-0.6162; p<0.001) and glycaemia and SFR (R=-0.5654; P=0.001). AgNORs number and status of maturation of mucosa cells were similar for all conditions. DM and DM+HAS presented the lowest SFR, which correlated with increased TGFβ1 levels. Despite the higher TGFβ1 secretion it was not observed changes in the morphology or proliferation of epithelial cells when diabetes or hypertension was present.
本研究旨在调查糖尿病患者和高血压患者唾液中转化生长因子β1（TGFβ1）水平以及上皮黏膜细胞的增殖/成熟情况。
在这项横断面研究中，收集了39例患者的全刺激唾液和口腔黏膜脱落细胞学标本，这些患者包括健康者（对照组，n = 10）、有动脉高血压病史者（HAS组，n = 9）、糖尿病患者（DM组，n = 10）或患有两种疾病者（DM + HAS组，n = 10）。评估了唾液流速（SFR）、唾液中TGFβ1水平、核仁组成区嗜银蛋白（AgNORs）以及上皮成熟情况。采用非参数Kruskal - Wallis检验，随后进行Dunn多重比较后检验以及Spearman检验相关性分析。与对照组（1.4±0.38 mL/分钟）相比，DM组和DM + HAS组的SFR显著降低（分别为0.47±0.11和0.64±0.43 mL/分钟）。DM + HAS组的TGFβ1浓度最高（24.72±5.89 pg/mL）。观察到TGFβ1与血糖之间呈正相关（R = 0.6371；p < 0.001），TGFβ1与唾液之间呈负相关（R = -0.6162；p < 0.001），血糖与SFR之间呈负相关（R = -0.5654；P = 0.001）。在所有情况下，AgNORs数量和黏膜细胞成熟状态相似。DM组和DM + HAS组的SFR最低，这与TGFβ1水平升高相关。尽管TGFβ1分泌增加，但在患有糖尿病或高血压时，未观察到上皮细胞形态或增殖的变化。
