Institute for Lung Research, Philipps-Universität Marburg, Hans-Meerwein-Strasse 2, 35043, Marburg, Germany.
Fachbereich Chemie, Philipps-Universität Marburg, Hans-Meerwein-Strasse 4, 35043, Marburg, Germany.
Angew Chem Int Ed Engl. 2018 Sep 3;57(36):11564-11568. doi: 10.1002/anie.201804090. Epub 2018 Aug 6.
Transgene expression of green fluorescent protein (GFP) has facilitated the spatiotemporal investigation of host-pathogen interactions; however, introduction of the GFP gene remains challenging in drug-resistant bacteria. Herein, we report a novel far-red fluorescent nucleic acid stain, 6-TramTO-3, which efficiently labels bacteria through a DNA binding mode without affecting growth and viability. Exemplarily, we stained Klebsiella pneumoniae, a major threat to hospitalized patients, and deciphered divergent interaction strategies of antibiotic-resistant and antibiotic-sensitive Klebsiella strains with immune cells. 6-TramTO-3 constitutes an off-the-shelf reagent for real-time analysis of bacterial infection, including strains for which the use of genetically encoded reporters is not feasible. Eventually, our approach may aid the development of strategies to combat a major worldwide health threat: multidrug-resistant bacteria.
绿色荧光蛋白（GFP）的转基因表达促进了宿主-病原体相互作用的时空研究；然而，GFP 基因的引入在耐药细菌中仍然具有挑战性。在此，我们报告了一种新型的远红色荧光核酸染料 6-TramTO-3，它通过 DNA 结合模式有效地标记细菌，而不影响生长和活力。举例来说，我们对住院患者的主要威胁——肺炎克雷伯菌进行了染色，并解析了具有不同抗生素耐药性和敏感性的肺炎克雷伯菌株与免疫细胞的相互作用策略。6-TramTO-3 是实时分析细菌感染的即用型试剂，包括那些使用遗传编码报告基因不可行的菌株。最终，我们的方法可能有助于制定策略来应对全球健康的主要威胁：多药耐药细菌。
