School of Biosciences, University of Kent, Canterbury, UK.
FEBS Lett. 2018 Aug;592(15):2543-2549. doi: 10.1002/1873-3468.13180. Epub 2018 Jul 13.
The ability to regulate polarised cell growth is crucial to maintain the viability of cells. Growth is modulated to facilitate essential cell functions and respond to the external environment. Failure to do so can lead to numerous developmental and disease states, including cancer. We have undertaken a detailed analysis of the regulatory interplay between molecules involved in the regulation and maintenance of polarised cell growth within fission yeast. Internally controlled live cell imaging was used to examine interactions between 10 key polarity proteins. Analysis reveals interplay between the microtubule and actin cytoskeletons, as well as multiple novel dependency pathways and feedback networks between groups of proteins. This study provides important insights into the conserved regulation of polarised cell growth within eukaryotes.
调节极化细胞生长的能力对于维持细胞活力至关重要。生长受到调节以促进必要的细胞功能并响应外部环境。如果不能做到这一点，可能会导致许多发育和疾病状态，包括癌症。我们已经对参与有丝分裂酵母中极化细胞生长调节和维持的分子之间的调节相互作用进行了详细分析。内部控制的活细胞成像用于检查 10 种关键极性蛋白之间的相互作用。分析揭示了微管和肌动蛋白细胞骨架之间的相互作用，以及蛋白质组之间的多个新的依赖途径和反馈网络。这项研究为真核生物中极化细胞生长的保守调节提供了重要的见解。
