[Wnt3a对牙髓干细胞成骨分化的影响]
[Effects of Wnt3a on osteogenic differentiation of dental pulp stem cells].
作者信息
Sun Y Y, Hu W P, Liu Z X, Wang W
机构信息
Department of Prosthodontics, Xuzhou Stomatology Hospital, Xuzhou Jiangsu 221000, China.
Department of Prosthodontics, The Second Affiliated Hospital of Harbin Medical University, Harbin 150086, China.
出版信息
Zhonghua Kou Qiang Yi Xue Za Zhi. 2017 Jul 9;52(7):427-431. doi: 10.3760/cma.j.issn.1002-0098.2017.07.007.
To investigate the effect of Wnt3a on osteogenic differentiation of human dental pulp stem cells (DPSC). DPSCs were subjected to different concentrations of Wnt3a (0, 5, 20, 50 and 100 μg/L) and at seven days after culture the alkaline phosphatase (ALP) activity was tested. Mineralized nodule formation was examined by alizarin red staining. Osteogenic-related gene expression of bone sialoprotein (BSP), osteocalcin (OCN), collagen type Ⅰ (COL-Ⅰ), Runt-related transcription factor-2 (RUNX2) was examined by quantitative real-time PCR (qPCR). After seven days of induction by DPSC, Wnt3a protein could inhibit the ALP activity (concentration 0: 1.076±0.203, 5 μg/L: 0.828±0.118, 20 μg/L: 0.505±0.044, 50 μg/L: 0.499±0.038, 100 μg/L: 0.483±0.060). The expression of OCN in 5 μg/L Wnt3a group (0.092±0.005) was lower than that in culture medium (0.858±0.190)(<0.05). Alizarin red staining showed that 5 μg/L Wnt3a had no mineralization induction effect on DPSC. Wnt3a could inhibit osteogenic differentiation of dental pulp stem cells.
为研究Wnt3a对人牙髓干细胞（DPSC）成骨分化的影响。将DPSC置于不同浓度的Wnt3a（0、5、20、50和100μg/L）中培养，培养7天后检测碱性磷酸酶（ALP）活性。通过茜素红染色检测矿化结节形成情况。采用定量实时PCR（qPCR）检测骨涎蛋白（BSP）、骨钙素（OCN）、Ⅰ型胶原（COL-Ⅰ）、 runt相关转录因子2（RUNX2）等成骨相关基因的表达。DPSC诱导7天后，Wnt3a蛋白可抑制ALP活性（浓度0：1.076±0.203，5μg/L：0.828±0.118，20μg/L：0.505±0.044，50μg/L：0.499±0.038，100μg/L：0.483±0.060）。5μg/L Wnt3a组中OCN的表达（0.092±0.005）低于培养基组（0.858±0.190）（<0.05）。茜素红染色显示5μg/L Wnt3a对DPSC无矿化诱导作用。Wnt3a可抑制牙髓干细胞的成骨分化。
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