Wang R, Liu F, Jiang N, Cai Y
Department of Periodontal Mucosa, The Affiliated Stomatological Hospital of Guizhou Medical University, Guiyang 550004, China.
Zhonghua Kou Qiang Yi Xue Za Zhi. 2018 Jan 9;53(1):13-19. doi: 10.3760/cma.j.issn.1002-0098.2018.01.004.
To investigate the expression of Epstein-Barr virus-induced gene 3 (EBi3) and interleukin-12p35 (IL-12p35) in two subunits of interleukin-35 (IL-35) in oral lichen planus (OLP) lesions, and to explore the role of IL-35 played in the formation and development of OLP lesions. Totally 41 samples of OLP lesions and 15 samples of normal tissues were collected from patients of the Department of Periodontology and Oral Medicine, Affiliated Stomatological Hospital of Guizhou Medical University from October 2010 to December 2016. The expression levels of EBi3 mRNA and IL-12p35 mRNA in the samples were detected by quantitative real-time PCR (qPCR) and the distribution and expression of protein EBi3 and IL-12p35 were detected by immunohistochemistry. The potential relationship between IL-35 and clinicopathological features of OLP was analyzed. The expression [((25), (75))] of EBi3 [3.38 (1.63, 11.25)] and IL-12p35 mRNA [6.39 (2.55, 14.30)] in OLP lesion tissues were significantly higher than those in normal control group [1.41 (0.33, 3.16), 2.47 (1.10, 5.14)] (-2.806, 0.005; -2.276, 0.023), respectively. The positive expression rates of EBi3 and IL-12p35 were 66% (27/41) and 39% (16/41), respectively, were significantly higher in OLP lesion tissues comparing with that in normal oral mucosa tissues [0%(0/15)] (0.05). The relative expressions of EBi3 and IL-12p35 were positively correlated (0.404, 0.009). A significant correlation was found between EBi3 protein over expression and the degeneration of base cells in OLP lesions (χ(2)=9.172, 0.010). The positive expression rate of IL-12p35 protein in erosive type lesions was higher than that in non-erosive type lesions (χ(2)=7.220, 0.007). The positive expression rate of IL-35 protein in OLP lesions [34% (14/41)] was higher than that in normal control group (χ(2)=6.829, 0.009). The expression rate of IL-35 in erosive type lesions (10/20) was significantly higher than that in eruption type lesions (4/21) (χ(2)=4.364, 0.037). The expression of IL-35 in OLP localized lesions was up-regulated, suggesting that IL-35 might play an important role in OLP lesion formation.
研究白塞病病毒诱导基因3（EBi3）和白细胞介素12 p35（IL - 12p35）在口腔扁平苔藓（OLP）病变中白细胞介素35（IL - 35）两个亚基中的表达情况，探讨IL - 35在OLP病变形成和发展中所起的作用。2010年10月至2016年12月，从贵州医科大学附属口腔医院牙周病科和口腔内科患者中收集41例OLP病变样本和15例正常组织样本。采用定量实时聚合酶链反应（qPCR）检测样本中EBi3 mRNA和IL - 12p35 mRNA的表达水平，采用免疫组织化学法检测蛋白EBi3和IL - 12p35的分布及表达情况。分析IL - 35与OLP临床病理特征之间的潜在关系。OLP病变组织中EBi3[3.38（1.63，11.25）]和IL - 12p35 mRNA[6.39（2.55，14.30）]的表达[（（25），（75））]明显高于正常对照组[1.41（0.33，3.16），2.47（1.10，5.14）]（-2.806，0.005；-2.276，0.023）。EBi3和IL - 12p35的阳性表达率分别为66%（27/41）和39%（16/41），与正常口腔黏膜组织[0%（0/15）]相比，在OLP病变组织中显著更高（P<0.05）。EBi3和IL - 12p35的相对表达呈正相关（r = 0.404，P = 0.009）。发现EBi3蛋白过表达与OLP病变中基底细胞变性之间存在显著相关性（χ² = 9.172，P = 0.010）。糜烂型病变中IL - 12p35蛋白的阳性表达率高于非糜烂型病变（χ² = 7.220，P = 0.007）。OLP病变中IL - 35蛋白的阳性表达率[34%（14/41）]高于正常对照组（χ² = 6.829，P = 0.009）。糜烂型病变中IL - 35的表达率（10/20）明显高于丘疹型病变（4/21）（χ² = 4.364，P = 0.037）。IL - 35在OLP局限性病变中的表达上调，提示IL - 35可能在OLP病变形成中起重要作用。
