Ndumbe P M, Levinsky R J
J Immunol Methods. 1985 Nov 7;83(2):337-42. doi: 10.1016/0022-1759(85)90255-8.
Sixty adults were tested for humoral and cell-mediated immunity to varicella zoster virus (VZV), type 1 herpes simplex virus (HSV-1) and the human cytomegalovirus (CMV). Since herpesviruses share common antigens, we compared results in these individuals to assess whether our tests gave false positives due to cross-reactions. Of the 60, IgG antibody to VZV, HSV-1, CMV tested by ELISA was detected in 51 (85%), 34 (57%) and 20 (33%) respectively. All possible permutations of results were obtained and there was no evidence of cross-reactivity among the viruses. Lymphocyte transformation performed in 20 was found to be positive in 16 (80%), 13 (65%) and 8 (40%) for VZV, HSV-1 and CMV respectively. Similarly, all possible permutations of results were obtained. In addition, 8 subjects were found to have positive lymphocyte transformation responses for one or other of the viral antigens but no specific antibody was found in their sera. Thus, the antibody and lymphocyte transformation test were not influenced by possible cross-reacting antigens among the 3 herpesviruses we studied. Both tests are necessary to firmly establish whether a subject has had a prior infection.
对60名成年人进行了针对水痘带状疱疹病毒（VZV）、1型单纯疱疹病毒（HSV - 1）和人巨细胞病毒（CMV）的体液免疫和细胞介导免疫测试。由于疱疹病毒具有共同抗原，我们比较了这些个体的测试结果，以评估我们的测试是否因交叉反应而产生假阳性。在这60人中，通过ELISA检测到VZV、HSV - 1、CMV的IgG抗体的分别有51人（85%）、34人（57%）和20人（33%）。获得了所有可能的结果排列，且没有证据表明这些病毒之间存在交叉反应。对20人进行的淋巴细胞转化试验中，VZV、HSV - 1和CMV的阳性率分别为16人（80%）、13人（65%）和8人（40%）。同样，获得了所有可能的结果排列。此外，发现8名受试者对一种或其他病毒抗原有阳性淋巴细胞转化反应，但在他们的血清中未发现特异性抗体。因此，在我们研究的3种疱疹病毒中，抗体和淋巴细胞转化试验不受可能的交叉反应抗原的影响。这两种测试对于确定受试者是否曾有过既往感染都是必要的。
