Wada M, Okamura H, Nagata K, Shimoyama T, Kawade Y
J Interferon Res. 1985 Summer;5(3):431-43. doi: 10.1089/jir.1985.5.431.
Infection of mice with Mycobacterium bovis BCG sensitizes them for immune induction of interferon (IFN)-gamma by specific antigen. These mice were found also to respond to lipopolysaccharide (LPS) and concanavalin A (Con A) with high level production of IFN-gamma in the circulation, which was not observed in control mice. In this study, we compared the IFN-gamma response to LPS with that to Con A in an attempt to clarify the cellular mechanisms responsible for in vivo LPS-induced IFN-gamma production. Consequently, (i) the responses to LPS and Con A differed in the kinetics, that to LPS having a longer lag period. (ii) Spleen cells taken from infected mice produced little IFN-gamma in response to LPS, but they showed a higher IFN-gamma response to Con A than those from control mice. (iii) By treating infected mice with immunosuppressive drugs or antibodies to T and natural killer cells before challenge with the inducers, it was indicated that different cellular systems are involved in the IFN-gamma responses to LPS and to Con A.
用牛分枝杆菌卡介苗感染小鼠会使其对特定抗原诱导免疫产生干扰素（IFN）-γ敏感。还发现这些小鼠对脂多糖（LPS）和伴刀豆球蛋白A（Con A）有反应，在循环中高水平产生IFN-γ，而对照小鼠未观察到这种情况。在本研究中，我们比较了对LPS和Con A的IFN-γ反应，以试图阐明体内LPS诱导IFN-γ产生的细胞机制。结果，（i）对LPS和Con A的反应在动力学上有所不同，对LPS的反应有更长的延迟期。（ii）从感染小鼠获取的脾细胞对LPS反应产生的IFN-γ很少，但它们对Con A的IFN-γ反应比对照小鼠的脾细胞更高。（iii）在用诱导剂攻击之前，用免疫抑制药物或针对T细胞和自然杀伤细胞的抗体处理感染小鼠，结果表明对LPS和Con A的IFN-γ反应涉及不同的细胞系统。
