School of Biology and Basic Medical Science, Agricultural Biotechnology and Ecological Research Institute, Soochow University, Suzhou, 215123, China.
Agricultural Biotechnology Research Institute, Agricultural Biotechnology and Ecological Research Institute, Soochow University, Suzhou, 215123, China.
Open Biol. 2018 Jul;8(7). doi: 10.1098/rsob.180029.
Hippo signalling represents a cell proliferation and organ-size control pathway. Yorki (Yki), a component of the Hippo pathway, induces the transcription of a number of targets that promote cell proliferation and survival. The functions of have been characterized in and mammals, while there are few reports on silkworm, In the present study, we found that facilitates cell migration and cell division, and enlarges the cultured cell and wing disc size. Co-immunoprecipitation results indicated that BmYki3 may interact with thymosin, E3 ubiquitin-protein ligase, protein kinase ASK1, dedicator of cytokinesis protein 1, calcium-independent phospholipase A2 and beta-spectrin. RNA-seq results indicated that 4444 genes were upregulated and 10 291 genes were downregulated after was overexpressed in the cultured cells. GO annotation indicated that the up/downregulated genes were enriched in 268/382 GO terms ( < 0.01); KEGG analysis showed that the up/downregulated genes were enriched in 49/101 pathways. These findings provided novel information to understand the functions of in a cell proliferation and organ-size control pathway.
Hippo 信号代表了细胞增殖和器官大小控制途径。Yorki (Yki)，Hippo 途径的一个组成部分，诱导许多促进细胞增殖和存活的靶基因的转录。 的功能已在 和哺乳动物中得到了描述，而关于家蚕的报道较少。 在本研究中，我们发现 促进细胞迁移和细胞分裂，并增大培养细胞和翅盘的大小。 免疫共沉淀结果表明，BmYki3 可能与胸苷、E3 泛素蛋白连接酶、蛋白激酶 ASK1、胞质分裂蛋白 1、钙非依赖性磷脂酶 A2 和β- spectrin 相互作用。 RNA-seq 结果表明，在培养细胞中转录本过度表达后，有 4444 个基因上调，10291 个基因下调。GO 注释表明，上调/下调基因富集在 268/382 个 GO 术语中（ < 0.01）；KEGG 分析表明，上调/下调基因富集在 49/101 条途径中。这些发现为理解 在细胞增殖和器官大小控制途径中的功能提供了新的信息。
