Di Corcia D, Salomone A, Gerace E
Centro Regionale Antidoping e di Tossicologia "A. Bertinaria", Orbassano, Turin, Italy.
Methods Mol Biol. 2018;1810:107-114. doi: 10.1007/978-1-4939-8579-1_10.
The determination at low concentrations of common psychotropic drugs is increasingly requested in hair samples for the retrospective investigation of habitual drug abuse and dependence as well as in other toxicological investigations. The dramatic improvements of the instrumentation based on liquid chromatography-tandem mass spectrometry (LC-MS/MS) make the detection of tiny amounts of almost whatever drug is in hair possible, even after single-dose intake. Therefore, LC-MS/MS is gradually replacing gas chromatographic techniques in both screening and confirmation procedures, and is increasingly acknowledged as the technique of choice for hair analysis. We describe a simple procedure for the quantitative determination in hair samples of 15 common drugs of abuse, or metabolites, based on methanol extraction and direct analysis by ultrahigh-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS).
在毛发样本中，越来越需要测定低浓度的常见精神药物，用于习惯性药物滥用和依赖的回顾性调查以及其他毒理学调查。基于液相色谱 - 串联质谱（LC-MS/MS）的仪器的显著改进使得检测毛发中几乎任何药物的微量成为可能，即使是单次服药后。因此，LC-MS/MS在筛查和确证程序中逐渐取代气相色谱技术，并越来越被公认为毛发分析的首选技术。我们描述了一种基于甲醇萃取和超高效液相色谱 - 串联质谱（UHPLC-MS/MS）直接分析，用于定量测定毛发样本中15种常见滥用药物或其代谢物的简单方法。
