State Key Laboratory of Biotherapy and Cancer Center, and Department of Neurosurgery , West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy , Chengdu 610041 , P. R. China.
School of Pharmacy , Shihezi University, and Key Laboratory of Xinjiang Phytomedicine Resource and Utilization, Ministry of Education , Shihezi 832002 , P. R. China.
ACS Appl Mater Interfaces. 2018 Jul 25;10(29):24469-24479. doi: 10.1021/acsami.8b10668. Epub 2018 Jul 16.
Clinically, postoperative adhesions are common and serious complications, which almost always happen after abdominal or pelvic surgery. The adhesion development process is accompanied by increased inflammatory cell infiltration and oxygen-free radical production. In this study, the naturally occurring antioxidative and anti-inflammatory compounds extracted from Turkish galls by ethyl acetate (GEA) were encapsulated into an injectable and biodegradable thermosensitive hydrogel. Antiadhesion efficacy of the barrier system (GEA-NP/H) was tested on a rat peritoneum injury-cecum abrasion model. Upon injection, the mildly viscous liquid formed a potent physical barrier over the injured cecum and peritoneum without any additional cross-linkers or light sources. Once formed, GEA-NP/H acted as a durable wound dressing for more than 5 days, as well as a sustained drug depot of GEA. The polymer hydrogel can be degraded and absorbed gradually. After 14 days, severe adhesion occurred among rats treated with normal saline and GEA-loaded nanoparticles (GEA-NP). Whereas, frequency of score 1 adhesion among the blank hydrogel group is 30%, and 90% of the rats from GEA-NP/H group exhibited no adhesion. In addition, pathological sections and scanning electron microscopy assay demonstrated that operative defects treated with GEA-NP/H suffered from mild oxidative stress and inflammatory damages at early days after injury, as well as accelerated wound healing and more mature mesothelial cell deposition at the 14th day in contrast to the blank hydrogel treatment. Therefore, the study provided an available biodegradable hydrogel barrier to effectively prevent postsurgical adhesion.
临床上，术后粘连是一种常见且严重的并发症，几乎总是发生在腹部或骨盆手术后。粘连的发展过程伴随着炎症细胞浸润和氧自由基的产生增加。在这项研究中，从土耳其五倍子中提取的天然抗氧化和抗炎化合物通过乙酸乙酯（GEA）被包封到一种可注射和可生物降解的温敏水凝胶中。屏障系统（GEA-NP/H）的抗粘连效果在大鼠腹膜损伤-盲肠擦伤模型上进行了测试。注射后，轻度粘性液体在受伤的盲肠和腹膜上形成了强大的物理屏障，而无需任何额外的交联剂或光源。一旦形成，GEA-NP/H 不仅可以作为一种持久的伤口敷料超过 5 天，而且还可以作为 GEA 的持续药物储存库。聚合物水凝胶可以逐渐降解和吸收。14 天后，用生理盐水和负载 GEA 的纳米颗粒（GEA-NP）治疗的大鼠出现严重粘连。然而，空白水凝胶组的粘连评分 1 的发生率为 30%，而 GEA-NP/H 组 90%的大鼠没有粘连。此外，病理切片和扫描电子显微镜检测表明，与空白水凝胶治疗相比，用 GEA-NP/H 治疗的手术缺陷在损伤后早期受到轻度氧化应激和炎症损伤，在第 14 天加速了伤口愈合和更成熟的间皮细胞沉积。因此，该研究提供了一种有效的可生物降解水凝胶屏障，可有效预防术后粘连。
